I am trying to find an indirect method/protocol for metallothionein estimation (non- radioactive method) in tissues. Please kindly help.
I have read similar papers where they have used Tukey's test and in some cases DMRT for one way ANOVA. What is the deciding factor for the type of test to be used for the study? Which is the best...
11 December 2015 4,431 11 View
I have recently got results for GC-MS & LC-MS of my plant extract. I am not sure how to interpret the results. I would like to know what compounds (molecular weight range) can be identified...
09 October 2014 5,201 29 View
Recently, many Journals are sprouting in the market. Each of these journals claim to have good impact factors. But unfortunately most are try to deceive the authors by false display of Impact...
09 October 2014 9,443 1 View
I am currently using AutoDock for docking my proteins and ligands. I am facing some problems with my step by step procedure (especially in the steps involved in preparing ligand PDBQT file. How to...
09 October 2014 2,766 24 View
I am not much familiar with DMRT using SPSS. An example with comparison of few groups and a set of 3-4 separate experimental data would be very helpful. For example: Groups 1,2,3 & 4....
09 October 2014 5,077 4 View
I know its not my field of expertise to talk about, but I came across two very interesting questions and I couldn't get a good answer for it. Can anyone tell me if "Heat" affects "Gravity"? My...
09 October 2014 7,365 3 View
Drying of plant extract
04 May 2013 5,296 11 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi, I know that low molecular weight (MW) molecules generally tend to have higher mobility, while high molecular weight molecules tend to have lower mobility. However, in my experimental...
06 August 2024 1,495 2 View
After immunohistochemistry of previously fixed in PFA and EtOH and then frozen 20 μm sections of zebrafish brain, DAPI staining is very weak (right) compared to the same sections stained without...
05 August 2024 9,637 2 View
Brain and body mass together are positively correlated with lifespan (Hofman 1993). The duration of neural development is one of the best predictors of brain size, and conception is the best...
05 August 2024 6,247 3 View
kindly reply me. Thanking you in advance.
05 August 2024 7,727 4 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View