How to implement monte carlo method to compute state value in matlab ?

assume there are 80 states each with a possibility of 2 actions. we have a predefined reward function. The problem is about research allocation.

30 October 2020 9,930 3 View

I am trying to use Modified cam clay as soil model in the simulation of an excavation problem in FLAC. I am getting an error. Can someone help?

The error says "Current value of tangent bulk modulus is larger than maximum input value". Input parameters were taken from a journal. When I try changing the bulk modulus, the error message is...

08 October 2019 5,267 3 View

What is the different between model, architecture, framework, and protocol in computer science?

I know this question is clear but I want to know the difference between these facts especially in computer science?

31 May 2019 5,710 2 View

Why the crack propagate in that direction (to the left hole)?

Left side of the specimen is applied with fixed support and load is applied downward on the right hole. I need an explanation why the crack propagate to left hole. What i have in mind is since...

18 February 2019 2,159 1 View

Why so called dark matter is outside the galaxy?

The so called dark matter is pictured out side the galaxy. What is the reason? why cant it be a part of the galaxy itself ?

03 September 2018 155 4 View

Can non-motile bacteria perform chemotaxis?

Most model for chemotaxis are flagellated bacteria. Im just wondering how this non-motile organisms adapt to starvation or repellent. If you can provide me some journal article that would be...

02 March 2018 9,880 3 View

Why high ROS signal in flowcytometry and fluorescence microscope?

Hello I want to investigate the effect of high temperature on ROS production in yeast. I have yeast cells which grow at 30 degree as a control and experiment cells grown at high temperature.I used...

21 April 2016 8,392 5 View

If two proteins run in sds page with different molecular weights, if both show bands with same intensity, does it mean they have same concentration?

If two proteins run in sds page with different molecular weights(having difference of 60 KDa),if both shows bands with same intensity,does it mean they have same concentration?

03 November 2015 9,304 3 View

Serum starvation in cell line treatment?

How long after seeding of cell line, should I do serum starvation or serum 4%? 24 h or less? How long should it last (before treatment) or serum 4%? 24 h or less? i i want to do serum starvation...

05 December 2014 8,834 3 View

In SDS PAGE, actin crosslinking doesn't show any significant increase in mass comparing actin bindings - any thoughts?

Can anyone help me to explain while running my protein samples in SDS PAGE, Actin crosslinking doesn't make any significant increase in molecular mass comparing actin binding protein samples.

29 August 2014 9,634 3 View

Protein-aptamer gel electrophoresis help, please??

Hi, I have problems with running gel electrophoresis. I have tried agarose gel electrophoresis and native PAGE. I have two proteins, which have molecular weights of ~30kDa and ~180kDa and two...

03 March 2021 4,275 4 View

Gel electrophoresis result for total RNA samples, 1.5 Agarose gel was used, How to improve the integrity of RNA?

Gel electrophoresis, RNA degradation, RNA extraction from fresh tissue

02 March 2021 5,433 5 View

How to regulate SSCP temperature in simple PAGE?

I'd like to perform single-strand conformation polymorphism (SSCP) in my thesis, however I cannot control the temperature of the vertical PAGE since we are using the conventional tanks. Is there a...

02 March 2021 9,157 1 View

Ester cleavage conditions?

Hi all, I need to cleave the ester in Ethyl 2-amino-1H-imidazole-5-carboxylate to make the carboxylic acid and retrieve the product from solution to use in downstream applications of linking to...

01 March 2021 8,766 3 View

Why are my bands weird in this Agarose gel?

Can someone please give me some possible things that could go wrong? Here is my recipe: 0.5g Agarose 50 mL of TAE 1x 1 uL ethyl bromide. Gel was run at 100V for 1 hour. The buffer used is also TAE.

01 March 2021 9,952 3 View

Precipitate after adding laemmli buffer to protein solution containing Potassium Acetate?

Hi, I am running a size exclusion chromatography experiment with a buffer containing Potassium Acetate as a salt. I analyse these fractions through SDS-PAGE. After boiling my SEC fractions in...

01 March 2021 2,622 3 View

Why my agarose gel staining sometimes looks not homogenic but like gradient (see the figure)?

I am worried about this overexposure of the upper part of the gel in the picture. Is it possible that this is the result of too much concentration of ethidium bromide? Why is there such a big...

25 February 2021 8,140 3 View

How to prepare a thin polymer film by post-polymerization/crosslinking?

24 February 2021 2,362 5 View

My silica gel column dried can I somehoe save the trapped matter?

I tried to separate my substance from a protein in a sillica gel column. By mistake, it dried and cracked. Can I save the trapped substance? Can I repack it ,or at least elute all of the trapped...

22 February 2021 1,789 3 View

Why my Total RNA samples do not have clear two bands?

This is gel electrophoresis result for total RNA samples with good conc. However, I guess there is a problem with the purity of the samples, i can't figure out why it appears like this? And how...

22 February 2021 338 9 View