Can anyone please explain how to quantify actin crosslinking in an acrylamide gel using any free softwares or any common methods ?
Is any validated questionnaire available to measure "House money effect" bias in the financial market (Likert scale)?
11 July 2024 6,772 4 View
We are using Vicon Blue trident IMUs for motion analysis. Nexus is used to stream data from IMUs currently, which are giving accelerometer, gyro, magnetometer and global angles. These data are...
04 July 2024 1,130 0 View
I am a PhD scholar looking for a Q1,Q2 Scopus indexed journal related to the domain of adolescent mental health and stigma, preferably with a good acceptance rate and quicker publication process....
07 February 2024 8,808 2 View
Hello everone. I'm very new in ML and DL models, and i want to use CNN to train and test it on my dataset, i had very large dataset and it is already split into 80% train and 20% test. I wrote...
06 February 2024 1,250 2 View
The arrangement is shown in the figure in the attachenment. One of the slit is having a glass slab in front of it. if photon is assumed as wave, then the part of it moving in the lower slit...
03 December 2023 5,349 1 View
I am trying to solve the system in the following figure by any numerical method. Can you please suggest me a source to learn this with Mathematica or Matlab?
02 August 2023 3,435 3 View
In some cases, it is difficult for a decision-maker to express an explicit approximation of the preference function. Therefore, it can be effective to allow the decision-maker to choose from a...
17 May 2023 7,484 0 View
Hi, this is Manjari here. I am currently pursuing a Ph.D. at Christ University. My research is titled “Development and Testing the Efficacy of a Mental Health Awareness and Destigmatisation (MHAD)...
24 August 2021 9,191 5 View
My name is Manjari; I am pursuing my Ph.D. at Christ University. I am conducting research titled "Development and Testing the Efficacy of a Mental Health Awareness and Destigmatisation (MHAD)...
05 May 2021 1,870 3 View
assume there are 80 states each with a possibility of 2 actions. we have a predefined reward function. The problem is about research allocation.
30 October 2020 10,169 3 View
I have been working on Red blood cell-derived extracellular vesicles as Antisense Oligonucleotide (ASO) carriers. We normally run agarose gel to quantify the loading efficiency. I used naked ASO...
06 August 2024 3,130 2 View
It's an end-point PCR protocol. I'm using 1.5% agarose gel with SyBR Safe dye and TBE as a running buffer, visualization on BioRad XR+ system. I was primarily thinking of primer efficiency,...
01 August 2024 4,673 4 View
Greetings. I’m currently running a nested pcr for giardia. My mastermix comprised of 3mM Mgcl2, 5unit of taq polymerase, 0.2uM of forward and reverse primers each respectively and 0.2mM of dNtp...
22 July 2024 9,761 5 View
I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...
22 July 2024 155 4 View
Sometimes I see the shadow like bands and its not true band. I want to know that what's the reason for it. I am using 2% gel for running genotyping samples I have uploaded the gel picture in both...
19 July 2024 148 6 View
I have been running native page for FAM DNA substrate ( fluorescence samples) for protein DNA binding reaction. Binding is there but towards the end of the lane , I am loosing signals...
17 July 2024 6,213 4 View
I start with IDT ordered 124 bp ssDNA that is PCR amplified, then I use a QIAgen agarose gel extraction to get my exact product, and I'm trying to purify ~95bp RNA by denaturing PAGE to prevent...
16 July 2024 4,604 0 View
I have conducted molecular marker amplification experiment by PCR and performed PAGE with silver staining. Now i have to score all my gels for further genetic analysis. Manual gel reading is...
12 July 2024 7,145 2 View
Commonly, we prepare 1.0% of agarose gel with 0.4g agarose powder and 40ml 1X TAE buffer. if I would like to prepare a 1.2%% of agarose gel, is it I need to add 0.48g agarose powder and 120ml 1X...
12 July 2024 2,058 5 View
I performed a nucleic acid extraction using 2 protocols for RNA extraction (Dengue Virus) and one parameter I need to evaluate is the integrity of my RNA samples. In this case, I can't use a RNA...
09 July 2024 4,857 2 View