21 September 2013 1 2K Report

I am going to start an experiment with certain nanoparticles to check their toxicity in a group of catfishes. I am wondering if anyone can explain me how should I check the LC50 value of those nanoparticles in those fishes?

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How to label synapses in over-fixed mice brain sections (40 um) via immunohistochemistry (IHC)?

We have mice brains that were over-fixed due to old PFA used during perfusion. Thus, the synapses are no longer being labeled by the Synaptophysin (SY38) mAB. which works perfectly every other...

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I see GFP signal on cells even though there is no GFP in them. Can anyone tell me what could be the reason?

These are iPSC derived human cortical organiods and I co-cultured them with tumor cells with GFP expression. After I fixed the tissues and amplified the GFP signal with an antibody. I would expect...

06 June 2024 2,289 3 View

Can anyone help me with GCF (Gingival Crevicular Fluid) elution from PerioPaper?

I am using PerioPpaer from Oraflow, to collect GCF, 8-12 PerioPapers from each subject and a pooled sample in PBS elution buffer. For the elution, I am vortexing for 1 min, then slow...

03 June 2024 9,767 0 View

How to cal culate the direction of dipolemonet for a molecule energy optimized using Gaussian package?

I performed single-point energy optimized using Gaussian software, followed by mapping the electrostatic potential surface around that. There is a clear dipole for that molecule but I want to...

24 May 2024 8,549 1 View

Could anyone kindly provide me the data regarding the variation of water flow rate for 24 hour horizon for small hydro power used in multi period OPF?

Optimal power flow, small hydro

20 May 2024 7,590 0 View

How to calculate the angular velocity of a rotating cell from a microbe video?

I have a video of Brownian motion of microbes under a microscope. From this video, I need to calculate the angular velocity of a particular prominent cell. From my understanding, what I know is...

18 May 2024 4,289 3 View

I have docked 17 antiviral drugs using UCSF Chimera AutoDock Vina. I need to select the best 3. How do I do that?

I need to select 3 best results from the list of the 17 antiviral drugs. How do I select that?

25 April 2024 5,386 1 View

What type of seagrasses and saltmarsh grasses are found in coastal odisha?

explain its morphology, taxonomy and habitat distributiion

03 March 2024 1,293 1 View

Can we extract and use only the sequences from the aligned regions for constructing phylogenetic tree and delete the unaligned sequences?

Suppose we are performing a phylogenetic analysis among different fungal species and we wanted to see how closely our species of interest is related with the other species across different...

28 January 2024 9,895 4 View

How to increase bootstrapping values of a phylogenetic tree?

I want to establish a phylogenetic relationship of our fungal species of interest along with the other fungal species belonging to the same order (Hypocreales). In order to perform this, we have...

22 January 2024 9,174 5 View

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Why results of ROS flurescence are negative as there was no bacteria within?

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