Bodhisattwa Banerjee

7 Questions 11 Answers 0 Followers

Questions related from Bodhisattwa Banerjee

Are there any alternative enzyme for reduction of glutathione other than glutathione reductase?

Glutathione is reduced mainly by glutathione reductase (GR). But in my catfish species we are not been able to identify/detect any gene sequence for GR. Although we have been able to...

03 September 2015 6,633 3 View

How to estimate the carbon present in microbial bio-mass wetlands?

I am going to work in carbon sequestration in wetlands. So could anyone share information about the standard method for estimation of carbon and nitrogen present in microbial biomass of wetlands?

27 June 2014 6,072 3 View

Does anyone have fish carbamoyl-phosphate synthetase III (CPS III) antibody?

I am working on the expression of ornithine-urea cycle genes and proteins in fish. But the problem is that we do not have fish-specific CPS III available commercially. So if anyone has CPS III...

09 April 2014 2,292 1 View

How can we reduce the frothing at the time of homogenising the tissue?

Whenever we homogenise the tissues for preparing samples for Western blot or any other experiment (i.e. enzyme assay, etc.), a lot of frothing occurs. How can we decrease the amount of frothing?...

24 March 2014 9,637 4 View

Does anyone have a primary cell line of walking catfish (Clarias batrachus)?

I am working in gene expression studies of walking catfish (Clarias batrachus). We use in vivo models for the treated conditions. But to study the inhibitor effects I need to have primary cell...

17 January 2014 9,374 2 View

Can anyone please explain how to determine the LC50 value of a certain nanoparticle?

I am going to start an experiment with certain nanoparticles to check their toxicity in a group of catfishes. I am wondering if anyone can explain me how should I check the LC50 value of those...

21 September 2013 2,089 1 View

Can anyone please explain why my qPCR results are showing exponential algorithm failed for a particular gene?

Whenever I am trying to run a qPCR analysis for a particular gene the exponential algorithm is failing. I have tried two different set of primers. Although the amplification curve is coming nicely...

01 September 2013 4,162 6 View