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Questions related from Bodhisattwa Banerjee
Glutathione is reduced mainly by glutathione reductase (GR). But in my catfish species we are not been able to identify/detect any gene sequence for GR. Although we have been able to...
03 September 2015 6,630 3 View
I am going to work in carbon sequestration in wetlands. So could anyone share information about the standard method for estimation of carbon and nitrogen present in microbial biomass of wetlands?
27 June 2014 6,067 3 View
I am working on the expression of ornithine-urea cycle genes and proteins in fish. But the problem is that we do not have fish-specific CPS III available commercially. So if anyone has CPS III...
09 April 2014 2,287 1 View
Whenever we homogenise the tissues for preparing samples for Western blot or any other experiment (i.e. enzyme assay, etc.), a lot of frothing occurs. How can we decrease the amount of frothing?...
24 March 2014 9,631 4 View
I am working in gene expression studies of walking catfish (Clarias batrachus). We use in vivo models for the treated conditions. But to study the inhibitor effects I need to have primary cell...
17 January 2014 9,365 2 View
I am going to start an experiment with certain nanoparticles to check their toxicity in a group of catfishes. I am wondering if anyone can explain me how should I check the LC50 value of those...
21 September 2013 2,083 1 View
Whenever I am trying to run a qPCR analysis for a particular gene the exponential algorithm is failing. I have tried two different set of primers. Although the amplification curve is coming nicely...
01 September 2013 4,156 6 View