Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000?

Is it possible to transfect cells with mRNA (less than 700bp) using Lipofectamine 3000? If yes- Is there some maximum number of bp of RNA, which could be transfected using Lipofectamine 3000? I...

08 November 2018 9,558 3 View

What is the optimal concentration of miRNA for microinjection into porcine oocytes?

Our stock is 5nmol. Or is it different for each miRNA? Thank you for answers.

30 May 2018 6,655 2 View

Someone who is able to statisticaly analyze MALDI-TOF data?

I am looking for anyone with experience with statistical comparison of MALDI-TOF data. I have no experience with analyzing profile data and I need to compare the effect of the treatment on...

19 September 2017 5,091 3 View

How to define multi volume heat fluxes within DFLUX subroutine, Abaqus?

24 May 2017 1,673 1 View

Why reporters of deviations in pharmacotherapy are not established and well paid in many European hospitals?

According to the media many fatal medical errors have been happened in many European countries. Although a 'soul' of inappropriate treatment and prescribing is well known in the literature, in a...

31 May 2016 2,834 8 View

Why and when should we combine or switch antidepressant in patients with major depressive disorder MDD?

02 January 2016 8,597 18 View

Why many Eastern European countries did not establish clinical pharmacy service in the hospitals next to the patients? Who will deal with errors??

According to the newest evidence more than 10 % of patients are admitted to the hospital, because of inappropriate prescribing and drug-related problems. More than 20 % of patients are treated...

11 October 2015 8,973 57 View

What is your opinion about quality of antibiotic prescribing within psychiatric wards and hospitals?

Prudent prescribing of antimicrobial drugs to hospital inpatients may reduce the incidence of antimicrobial drug resistance and healthcare-associated infection. Despite strenuous efforts to...

24 March 2015 3,714 14 View

Are there any studies showing age related hypertension in regular strains of mice?

In particular I'm interested if hypertension occurs in C57BL6 mice at any time after their 18th month of age. But I am also interested in other strains. If you could point me to such studies I...

04 March 2015 401 6 View

Is there a typical value for the birefringence of a PDMS film?

I am interested in the birefringence value since it is needed for film thickness determination using the Michel-Levy Birefringence Chart. How could one measure the birefringence of a thin PDMS...

30 January 2013 4,322 1 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

MD MOE RMSD

How to calculate the RMSD values for a MD simulation using MOE?

07 August 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

fatal error

When I tried to energy minimization my system, I got fatal error as below. Fatal error: Atomtype opls_116 not found Although I've already added this line: ; include water #include "oplsaa.ff/spc.itp" to [molecultype] directive in my topology.

16 June 2021 0 0 View

flexibility of polymers chain

effect of double bond on the flexibility of polymers

07 April 2021 0 0 View

Formulation of polymer color flakes

I am writing to request some information for making polymer color flakes. I would appreciate the help.

17 March 2021 0 0 View

How to compare two groups with only two measurements?

Hiiiii everyone! I have an enquiry on statistical analysis. I was looking for many forum and it's still cannot solve my problem. I want to compare means of two groups of data but only with two...

03 March 2021 8,796 3 View

Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence?

I am on the lookout for the Enhanced Yellow Fluorescent Protein (Aequorea victoria) DNA sequence. Does anyone know where I can find it? Thank you in advance

03 March 2021 3,568 1 View