I am surprised that so many suggested elaborate test when you can get for much less effort and money / time the definite answer using 16S rRNA sequence (Cost of sequence is about US $ 5 -29 , depending on the service, If the sequence is between two species you can do --based on Bergey's-- very few differentiating tests to nail it down. Don't forget you also could have a novel taxa and you don't find that out by just doing the suggested test.. Don't think in a box. -

You can always send a plate to SeqWright in Houston. For $100 they will identify it via 16S rRNA sequence.

Pseudomonas

It seems Pseudomonas spp.

I would vote for Pseudomonas as well, in particular, P. aeruginosa. Are cells rod-shaped and motile (I assume you've looked at the culture under the microscope?)

Pseudomonas aeruginosa or Pseudomonas fluorescens, depending on biochemical profile. Knowing where it was isolated from would help. Burkholderia cepacia is a less likely hypothesis.

I thought Pseudomonas as well

Most Probably Pseudomonas spp.

Is it pseudomonas spp

Only pseudomonas spp produce blue green pigment that to in an basic media like nutrient broth

It seems to be Pseudomonas spp.

Pseudomonas for sure

Yes, it is most likely to be a Pseudomonas (but Proteus may also produce some pigment).

Pseudomonas

I recommend the following site for help, good luck.

http://rtsf.msu.edu/

Pseudomonas for sure. Try to observe the preparation under a Wood's Lamp to see if the pigment is fluorescent

Pseudomonas species

Where was it isolated from?Infection/ enviromental sample? That information might greatly contribute to the right identification. As aforementioned, I also strongly recommend you DNA extraction and further sequencing of 16S rDNA amplicons. Identification based on sequencing helps to avoid misleading results associated with variable expression of some phenotypic traits depending on strain or cullture condition e.g. carbohydrate fermentation or other metabolic capabilities. Good luck!

yes i agree the last opinions especially with Mike Mitzel because this the right way to classify any strain. also you have to do one PCR run using the specific primer. Hence you will be sure that you have pseudomonase strain or what.....good luck

ya this may be Pseudomonas sp if you want to confirm check in pseudomonas agar if it grows it wll confirm as Pseudomonas while checking see the fluoresence if it shows fluorescense it is confirm Pseudomonas sp

Perform genetic analysis.

I suggest to use API method to identify your Bacteria.

90% Pseudomonas, but you need to confirm, ie to perform steps Mike Mitzel

looks like you have genus Pseudomonas.

Mike Mitzle- could be pyoverdin production, not pyocianin,

Pseudonomas are all oxidase positive and motile. They all can oxidize glucose but not ferment it.

I suggest you send your plates to a contract lab for microbial identification, you may need to know the genetic aspects of all microorganisms identify or you can do it yourself by using biomerieux API kits or Remel.

Contract labs: MIDI or accugenix

Regards,

Suze Philistin

partial sequence of the 16S rDNA for genus, then genotyping comparing with typre strains

Pseudomona smells like old soap.

i am quite sure it is Pseudomonas, and that bluish compound is pyocyanine a redox active compounds, and serve as quorum sensing molecule

More likely pseudomonas

on first look seems like Pseudomonas but should be confirmed

Probably Pseudomonas to confirm 16S rDNA ; you can send the strain at DSMZ Germany for the identification.

Can you post a pic of colony characteristics on plate?

You can get the genus by doing 16srDNA sequencing, then if you submit the sequence in NCBI blastn databases, it will give you the possible matches of the genus. like this by biochemical characterization you can't be confirmed 100%.

I also agree that 16S rDNA sequencing can solve your problem.The Pseudomonas genus is one of the most diverse genera. It is a bit tricky to identify Pseudomonas species based on biochemical tests and cultivation on different media. If you have possibility try to do the 16S rDNA sequencing (at least the parital sequencing).

One thing I can tell you that P. aeruginosa is urease negative...so your culture might be some other species of Pseudomonas..You should check the family pseudomonadaceae in Bergey's Manual of systematic bacteriology and match your results of biochemical tests..

Mr. Dipak Paul

if you could send a stab culture or culture pellet in ethanol then I may try to identify both genus and species

What are its morphological features, both on plate & under the microscope?

Probably it is a specie of Genus pseudomona. Why don´t you test oxidase? If it is oxidase negative, then it is most likely to be Proteus and you can test for swarming in nutrient agar.

@ Ruhi sounds quite correct but needs verification with the isolate

Thanks to all of you!!

Mr.Dipak Paul first send the details about colony morphology, source of bacterium, gram's feature, oxidase, if gram negative, whether you have checked or confirmed with 3% KOH, then all biochemical tests performed.

send details to guess the bacteria Thanks

Fam Pseudomonaceae: Gram - oxidasa + catalase +

Gen Pseudomona: gelatinasa + ureasa +

Pseudomona aeruginosa: (Bacilo piocianic) Can reduce NO3- to NO2-

High resistance. (Use polimixine- Gentamicine).

i think it is a other species of Pseudomonas

You should continue your biochimocal test , you are in Peudomonadaceae

What is the Gram reaction and what is the shape of the bacteria when seen under the microscope? Not all green pigment producing bacteria are Pseudomonas sp

One quick way of identfication would be to run teh bacterial colony though a MALDI-TOF MS.

Could you tell me, what was the response to oxidase?

Any info on gram stain....will see where it belongs....

looks as some species of Pseudomonas

If it is possible to have a sample of this bacteria, we have a Biolog system to identify it to you. We can also identify it by the DNA sequencing.

Best regards

16S rDNA sequencing should give a definitive identification. That would be my choice. Good luck!

So Dipak..have you been able to identify the bacteria?

Dipak you just go for FAME analysis once you got the result you just compare with 16S rDNA sequencing data it probably will give the better result of your bacteria.........

If it is gram negative rod polarly flagelated bacteria, it might be a Pseudomans species???

better u ill try 16s r RNA sequence

I am late but genus might be Peudomonas pls check in COWAN and STEEL identification of bacteria manual

it must be Pseudomonas ,species not exactly aware of

Is the pigment fluorescent? What is the source of the culture - I have seen similar organisms in marine samples.

Source is river water.

grow this culture in argenine proline broth with ethanol if growth occur then it is Psedomonas aeruginosa................

yeah its maybe Pseudomonas sp, Pseudomonas fluorescence maybe

Is it gram positive or negative?

16s korechis???tahole e to jante parbi ki genus...species ta ektu chaper bapar...otr jonno onek onek khatte hobe.. :-)

It looks like Pseudomonas but if want to identify up-to species level better go for sequencing.

Pseudomonas aeruginosa

Dear you are talking about it's genus then 99% it might belong to Pseudomonas sp.

Properties so far suggest it is one of the Pseudomonas species. Sill 16S rRNA sequencing may clear the facts.

It coud also be Azospirillum.... once got similar thing when isolating Azospirillum lipoferm from paddy fields.....

What is the oxidase response? Does it ferment lactose? I'm also inclined to the family pseudomonadaceae; Oxidase should test positive to this.

This bacterium is certainly Pseudomonas,Melt gelatin tests, the sugar trehalose, growth at temperatures of 41 and 4 ° C. is major indexes for Separation of the Pseudomonas species, But I think it is Pseudomonas aeruginosa.

A few points to note.Some strains of Pseudomonas aeruginosa can be urease positive.However, definitely NOT H2S producers..

Rod ? cocci ? Gram +, Gram - . These microcscopic characteristics would be very useful here.

It's a rod G- Oxidase -? You need to check first this point!

you need to follow a systematic approach to the identification, colony morphology, microscopic morphology, gram stain, primary/secondary biochemical tests. Then followed by the specific genus/species tests. This would be the standard approach that all science student are shown in year one. Use a good book, eg Bergies Manual to perform the identification. Unless you do this your isolate could be anything.

I also think you should use a systemaytic approach for the identification, or if you can you could go for a 16S seqeunce whcih gives you really good informations. But I think that this bacterium is close related to some Pseudomonas species. The coloration could be due to phenazine production, check this paper http://www.nature.com/nchembio/journal/v2/n2/abs/nchembio764.html

Best

as per your biochemical test it may be Pseudomonas..better your try to sub culture it on Kings B agar which is selective medium for Pseudomonas. and try with Cetramide agar too..for better confirmation 16S rRNA sequencing is best option

I am not answering the question, but I wish to comment on the right use of the word bacteria. The pharse "this bacteria" should read "this bacterium".

It is a Pseudomonas.... You can confirm with phylogenetic marker 16s rRNA gene

I'd say just amplify the 16S rRNA genes and sequence them. Nadkarni et al 2002 describe the primers and conditions needed.

Hi Dipak, thanks for posting this question. Your bug appears to be a Pseudomonas to me also.... though a confirmatory test would be better. Depending on your time and available funds you can try to identify your bacterium. If you have funds for a 16S rRNA analysis, please go ahead and do that (you can reach the IISER in kalyani; IICB/ICGEB/IMTECH/CCMB/NCL/NIO/IITKGP/TIFR/ and many more institutes in India itself who can do it for you in less than 3k INR). If you have time but lack of funds, please grab the Bible for microbiology (Bergey's manual ), carry out some (may be lots too) biochemical tests, and you should be done with the identification unless you have a new bug that would be very difficult to funnel out from those tests and charts... Good luck...:-)

The pigment looks like pyacyanin which would mean you have a urease positive strain of P.aeruginosa!

I am surprised that so many suggested elaborate test when you can get for much less effort and money / time the definite answer using 16S rRNA sequence (Cost of sequence is about US $ 5 -29 , depending on the service, If the sequence is between two species you can do --based on Bergey's-- very few differentiating tests to nail it down. Don't forget you also could have a novel taxa and you don't find that out by just doing the suggested test.. Don't think in a box. -

yes i also agree with Sir Wiegel, you have to go for 16S rRNA sequencing, then you can easily determine its phylogenetic related species.

hi

It may be Pseudomonas.fluorescence .Because it got the ability to produce both pyoverdin and pyocyanin ,And this microbes got little effect on phosphorus soliubilization around (0.6-0.8 ) PPM. For more details go for inhibition tests as it is effective against rice sheath rot disease .....All the best

Feel free to contact me

Abiram

hi,it is certaeily P.aeruginosa my dear

p. aeruginosa confirm on cetrimide medium it is specific for it. always use Bergyes manual

H2s and urease are against pseudomonas

You have not mentioned about oxidase pseudomonas are positive