Can anyone suggest the necessary column and buffers for purification of a fungal Isoamylase? Should I choose DEAE- Cellulose or Sephadex A50 and why?

It has been studied by us that this Isoamylase is readily adsorbed onto raw starches such as arrow-root powder, corn flour and also kaolin powder, thereafter being easily eluted by cooled...

03 March 2013 483 0 View