Does anybody have experience visualizing intestine whole mounts by confocal microscopy (IF) and could give me some details about the protocol to get clear images of crypts and villi?. In my hands, the tissue is too thick and antibodies cannot penetrate so well, besides the fact that is very difficult to focus on the microscope.
Hi,David, Have you got a good protocol for the small intestine whole mount staining? I am also finding a protocol for this. If you resovle this problem, can you give me some advice? Thank you very much.
Hi David Fernandez-Antoran and Chen Shiyang
Hope you got a good protocol for whole mount staining on mouse intestine? I am more interested toward the isolation of intestinal layer for doing immunostaining on myenteric and submucosal plexus on mouse colon.
Thanks in advance
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