Chen Shiyang

2 Questions 4 Answers 0 Followers

Questions related from Chen Shiyang

Did anyone culture the mouse small intestinal T cells in vitro?

I want to culture the mouse small intestinal T cells in vitro. But I don't know whether I need to add any cytokines for the T cell activation and maintenance.Whether the T cells from gut have been...

19 July 2016 809 1 View

The intestinal crypt organoids I cultured in the Matrigel don't bud, is there anyone that encountered such a case?

Recently there is some trouble in the 3D intestinal crypt culture system I built. The crypts I isolated from the mouse intestine didn't bud and grow to form the mini-gut structure. They just...

15 January 2016 6,945 6 View