How to extract gene expression data from the Phylofish database for a specific gene?

I am having trouble navigating the phylofish database. I can blast my gene of interest and get the " Best match," but from there, I cant seem to figure out where to go to find the expression level...

15 September 2020 4,882 2 View

What is the recommended way to store rice seed for long term viability?

I work in a plant biology lab on a rice development project, but as far as I know I'm the only person at my university working on rice. As a consequence I don't have a lot of institutional...

17 June 2020 9,434 1 View

How do other researchers measure relative quality control in production of ancient Glass?

I find it interesting that there is very little emphasis on uniformity of production when it comes to early glass manufacture. To me, it is an essential ingredient when analysing the differences...

02 June 2020 2,724 1 View

How to calculate error in photocatalytic data?

Hello, I made some measurements using a UV-vis spectrometer at intervals of 5 minutes to see the degradation of a resazurin dye by TiO2 under UV light. I did not repeat these measurements. How can...

23 May 2020 1,373 3 View

How to measure extraversion and introversion?

Hello! I'm wondering if anyone can help me. For my masters dissertation, I am looking how personality, particularly extraversion and introversion, may impact on responses to several communicative...

13 May 2020 4,500 6 View

Do rats play a part in Covid-19?

The VIZIONS project in Vietnam in 2018 examined bat and rat urine and faeces. Alpha coronavirus was found in bats, beta in rats, with a comment that rats in China also carry coronavirus. So,...

10 May 2020 7,974 5 View

How do I combine two dummy variables to one variable in SPSS?

Hi everyone, I want to combine two variables in spss, one is Twitter Use measured by yes/no with yes being 1 and no being 2. The other variable is Facebook Use measured by yes/no with yes being 1...

26 March 2020 6,476 2 View

What scale of measurement would I give to this variable?

Hi everyone, I am trying to determine what scale of measurement to give to this variable: On how many days of the week do you discuss Politics with people? The answers that people can give are:...

26 March 2020 6,529 4 View

What is the level of measurement of Likert scale data for variables in SPSS?

Hi everyone, I am writing my dissertation and I am very confused on how to measure my variables. When opening my spss data it all says unknown. One variable is: Interest in politics. The...

25 March 2020 3,426 30 View

How to model a hyperelastic plate in ansy apdl?

I am modelling an elastomeric bearing in ansys apdl but the elastomer does not bulge under compressive load as it should. it "splashes out" under any significant loading. is it the loading problem...

26 June 2019 2,003 3 View

Waters 2707 HPLC autosampler problem?

I am using a 2707 waters HPLC device. When I try to inject a sample, it says missing plate or rack. I changed the needle and calibrated its position but I still get the same problem. I even get...

02 March 2021 1,408 1 View

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02 March 2021 3,060 3 View

Should we change media after1st 6hrs after plating HUVEC bought from INVITROGEN?it's not recomended by invitrogen.Will DMSO present in stock matter?

We have HUVEC bought from Invitrogen. Cat no -C-003-5c. while first plating the cells we have bought the protocol suggests not to centrifuge the cell with media to remove the DMSO. Should we...

02 March 2021 3,713 2 View

How to normalise data on OPM package from R Studio ?

Good afternoon, I recently used OmniLog from BIOLOG for my experimentations : I tested the metabolism of different strains on 2 types of plates. I have 16 strains of 3 different groups...

02 March 2021 3,584 1 View

How do I determine the degree of polymerization of PEG 400 based on NMR integration?

Hello, I am working on the photocatalytic degradation of PEG 400. I would like to know if it's possible to determine the degree on polymerization based on NMR integration, or by the MW of the...

02 March 2021 6,186 3 View

Do you know any MR image dataset?

I would like to research on MR images (0.5T and 3T). Can you please suggest some websites that I can download dataset including both 0.5T and 3T MR images? Thank you.

02 March 2021 7,735 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View