How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Binary Logistic Generalized Linear Mixed model in SPSS. Is it doable?

I want to analyses the proportion of swimming sperm of three species of fish in two salinities. To analyse the proportion of swimming sperm in a Generalized Linear Model, I would use a Binary...

03 March 2021 2,297 3 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

Guidelines for survey (question type) analysis?

Hi. Please tell me what guidelines should i need to follow for questionaries' type research work in India. It is not hospital based work, we are conduction basic institutional based qualitative...

03 March 2021 2,037 3 View

What are the values needed for gabor filter's parameter for cifar10 data?

Hi, I implemented a code to gabor filter cifar10 data but the images after being filtered and stacked are not clear like the original images. I think the problem is in the way I am using the...

03 March 2021 6,317 1 View

Can we block unwanted images during the x-ray prediction?

i am try to classify the x-ray images. During classification , can i block unwanted images (except x-ray image).

03 March 2021 7,100 1 View

No title

03 March 2021 5,360 2 View

What techniques can I use to determine the miscibility of polymer blends?

The term miscibility refers to the single-phase state in thermodynamics. I do not mean the compatibility of different components. To determine the miscibility I know several techniques such as...

03 March 2021 4,107 4 View

Sample dilution for quantitative analysis using ELISA?

If the detection range is in ng/ml but the reference range is in ug/ml for a molecule or protein in serum or plasma .how to dilute and what is the initial volume to be taken for quantitative analysis

02 March 2021 7,670 3 View

The procedure compute?

02 March 2021 5,204 3 View

How do I quantify lead in tissue?

Hello everyone! I'm looking for some novel ways to measure out the amount of lead shot spray found in wildlife tissue. I have radio-graphed some deer ribs and gut piles left by hunters. They are...

24 January 2021 2,150 7 View

Is it worth to publish book with self-publishing agencies/publishers?

It is observed that most self-publishing publishers are active in the publishing sector/market. Browsing through such publishers' portal is sometimes quite impressive. They published a good number...

30 October 2020 9,852 5 View

Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 9,943 3 View

Plasmid linearization for in vitro transcription?

Hello, We would like to in vitro generate mRNA from this plasmid, encoding SB100X transposase https://www.addgene.org/browse/sequence/178440/ using mMessage mMachine T7 Transcription Kit. We...

10 November 2019 8,695 3 View

Is this ISI indexing site legit or scam?

So I was just browsing the net for journal publishing site. Eventually come across this website: https://isindexing.com/isi/ I need a conformation if its legit or not. Because the application to...

25 September 2019 7,640 3 View

Is this ISI indexing site legit or scam?

So I was just browsing the net for journal publishing site. Eventually come across this website: https://isindexing.com/isi/ I need a conformation if its legit or not. Because the application to...

08 September 2019 8,382 4 View

How to handle gap in GPS data on same individual?

I am using GPS data to conduct seasonal habitat and survival analysis on deer. I have an individual that has an 8 month gap in between collar fixes due to a collar malfunction. The collar...

25 July 2019 1,023 3 View

How to handle gap in GPS data on same individual?

I am using GPS data to conduct seasonal habitat and survival analysis on deer. I have an individual that has an 8 month gap in between collar fixes due to a collar malfunction. The collar...

06 July 2019 4,014 3 View

Can any body guide me??

I want to work on scientific investigation (Ecology+Genetics) and community based conservation of two threatened species i.e. the Himalayan Gray Langur and the Himalayan Musk deer in western...

06 July 2019 9,653 6 View

How to make Meta Analysis in Engineering professions?

Recently I have browsed to a web page " https://www.ncbi.nlm.nih.gov/pubmed " which supports to search, filter, and give detail information about the searching details. I wounder if there is any...

24 March 2019 9,432 10 View