All good over here, don't mind if I answer my own question.

We bought a Biorad electroporator and have gotten good results thus far.

Olivier,

We have Biorad too and it gives good results with some cell types but not others. The advantage of this unit is that the discharge parameters are defined by the user and can systematically modified. The 'black box' electroporators don't allow you to do that, only to try pre-defined programs.

Hi Ashot, we met several years back at Wright State!

Yes, I am hopping to get better than 18% expression, at most, in PC12 cells. Maybe we can share parameters.