18 February 2014 6 1K Report

Keratin is an expensive product, so I would like to extract it and use for my experiments.

More Vivek Narayan Upasani's questions See All
Can anyone identify the tick species ?

Can anyone tell me the tick species

13 July 2024 7,146 6 View

Journal's Impact Factor: Publication or Updated?

There are two scenarios: The Impact Factor at the time an article is published. The Impact Factor after the journal updates its metrics annually based on performance. While being interviewed or...

25 June 2024 7,825 2 View

Can anyone explain how mobile ions moves inside ploymer electrolytes?

I want to know the exact mechanism behind the motion of ions inside the polymer electrolytes.

13 June 2024 3,489 0 View

Funding sources in India to attend international conferences (in abroad)?

Being a Ph.D. Scholar, I am seeking funding sources that offer financial assistance for conference registration and travel expenses to attend international conferences, specifically those held...

26 May 2024 712 2 View

I'm facing serious problem about sample size determination please help me?

I'm doing research in veterinary science and topic is associated with ticks prevalence and seasonal variation in cow..I have randomly selected 560 cows as sample size and studing for prevalence...

25 May 2024 3,834 5 View

SHA-256 Hash function transmission?

I have applied the SHA-256 hash function to the plain image to generate the initial values of the chaotic map in the image encryption algorithm and include it in key space analysis. So, when the...

10 March 2024 8,854 4 View

I am getting higher seismic demand in terms of displacement for FRP confined RC column as compared to RC column?

I am using concrete01 and the stress-strain parameter is calculated using Lam and Teng model for FRP confined concrete. How to correct the model so that demand for FRP retrofitted will become less.

05 March 2024 7,328 0 View

Rapidly publishing SCI journals with low impact factor?

Area: Manufacturing, additive manufacturing, CNN, mechanical engineering

09 February 2024 7,943 3 View

How to export RGA data in Partial Pressure (PP) unit in FabGuard explorer which is used for INFICON XPR3 RGA?

I am using Inficon Transpector XPR3 and fabguard explorer software to analyze gases in our vacuum system. I am facing an issue in exporting the RUN data in other units. I can see that I can switch...

24 January 2024 5,303 1 View

Benefits of Wohler's report 2023?

Has anyone gone through the Wohler's report_2023 yet? Its pros and cons? What are the ways to obtain its e-copy? Its subscription is very costly for a normal researcher (around 750 USD per user)....

15 January 2024 8,303 2 View

Similar questions and discussions
Is there a problem with my RNA pellet?

Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...

11 August 2024 7,082 3 View

RNA Extraction Using Hot Borate Method No Longer Working?

I've been performing RNA extraction on cotton petiole tissue for a few months now using the method described in the following paper, a derivative of the typical hot borate method...

08 August 2024 9,882 2 View

Low-yield gel extraction problem?

I am having an issue with my gel image where my PCR product is not appearing very bright on the gel. When I perform gel extraction, the A260/280 purity value is very low. I used the Qiagen gel...

05 August 2024 9,798 3 View

Do you have good tips for seaweed tissue preservation in the field for post RNA extraction?

I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...

04 August 2024 501 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

The question is how to use Wavenet transform?

HOW CAN I WRITE A CODE TO USE THE WAVENET TRANSFORM AS A FEATURE EXTRACTION METHOD INSTEAD OF DWT IN MATLAB?

03 August 2024 7,829 0 View

Can i use the protease inhibitors during cell membrane vesicle preparation ？?

I am currently working on a project that involves extracting cell membranes, for which we disrupt the cells using sonication. During the initial extraction process, we add protease inhibitors to...

30 July 2024 7,077 1 View

Pink bacterial colonies?

I am cloning an overexpression plasmid with my protein of interest tagged with mScarlet. After transforming my ligated product into DH5α bacteria and plating on LB agar, I noticed colonies with a...

22 July 2024 5,953 6 View

How should we increase the quality of RNA extraction?

I’m having difficulty achieving high RNA integrity in my samples. Although the 260/280 and 260/230 ratios are satisfactory after RNA extraction, the RNA samples show signs of degradation when...

22 July 2024 155 4 View

The best source for amplification of ADAM17 prodomain?

hi every one I am making vector construction (for fusion proteins) and in this moment I wanna to amplification of ADAM17 prodomain with PCR. to yet, I couldn't amplified the ADAM17 prodomain with...

21 July 2024 8,660 1 View

Our partners will collect data and use cookies for ad personalization and measurement. Learn how we and our ad partner Google, collect and use data. Agree & close