Vijay Kothari

12 Questions 31 Answers 0 Followers

Questions related from Vijay Kothari

Which lifecycle stage of C. elegans is most amenable for RNA extraction?

For gene expression/ transcriptome analysis, which stage of the life cycle of the worm C. elegans (L1-L4 or adult) is most suitable for isolation of good quality RNA? If RNA isolation is done from...

19 May 2025 1,917 4 View

Is there any free tool for worm counting?

I have videos of C. elegans moving in 24-well plate. Is there any free software available which can analyze these videos to quantify worm movement and/or live-dead estimation? If someone knows,...

07 January 2023 5,209 2 View

Protocol for Nitric Oxide Reductase (NOR) activity?

We want to estimate NOR activity in Pseudomonas aeruginosa. Can anyone share a protocol for the same? A protocol based on UV-vis spectrophotometry would be more likely to be executed in our lab...

23 May 2022 6,090 0 View

Can Prevotella bacteria be cultivated without anaerobic jar?

The genus Prevotella comprises of anaerobic bacteria. What I want to know is whether they are strict anaerobes, or can tolerate oxygen. Is it necessary to have a atmosphere rich in CO2 to...

16 September 2016 6,594 1 View

How 'dose independent response' observed during a susceptibility assay can be explained?

Sometimes, while screening natural products for antimicrobial / anti-infective potential, the results do not show any pattern; i.e. with increase in concentration either there is no increase in...

12 February 2016 7,927 2 View

Are bacterial pigments other than pyoverdine known to bind iron?

Pyoverdine is a known siderophore. What about other pigments, like prodigiosin, pyocyanin, staphyloxanthin, violacein, etc.? Can they chelate iron?

12 February 2016 8,422 1 View

Is there any association between stress response, and pigment production in bacteria?

Can higher pigment production in bacteria (e.g. Serratia marcescens, Staphylococcus aureus, Pseudomonas aeruginosa, etc.) in presence of a particular compound(s), be taken as an indication that...

11 February 2016 9,197 2 View

What may be the effect of quorum-sensing inhibitors on normal microbiota?

Currently there has been a considerable interest in screening natural products for their potential quorum-sensing inhibitory potential. Though these inhibitors can be effective therapeutic agents,...

04 August 2015 259 4 View

While taking OD of a microbial suspension, if OD values are more than 1, is there any problem with reliability of data?

I am talking about the digital spectrophotometer (and not the analogue type). Theoretically OD = 2 - log T, thus max OD can be 2. While quantifying microbial growth at 625 or 660 nm, if the OD...

07 April 2015 3,940 1 View

Can anybody suggest a simple method for estimation of membrane permeability in bacteria and yeast, citing a relevant reference?

We are looking for a simple method which does not require any sophisticated instrumentation. I think there is some method wherein some substance of known molecular weight can be added into the...

11 July 2014 9,516 3 View

If some bacteria is not forming good biofilms on the surface-treated polystyrene plates, then what other options can be tried?

Surfaces like glass can be tried, but it is usually convenient to work in multi-well plates, so I would like to know whether any modification in media etc. can help. Addition of sucrose, we have...

27 June 2014 9,209 3 View

How can we restore the biofilm forming capacity of a Streptococcus mutans strain, which it has lost due to some unknown reason?

One S. mutans culture with us was forming good biofilm until recently. But since last few experiments, its biofilm formation is not satisfactory.

27 June 2014 7,110 4 View