10 Questions 26 Answers 0 Followers
Questions related from Vijay Kothari
I have videos of C. elegans moving in 24-well plate. Is there any free software available which can analyze these videos to quantify worm movement and/or live-dead estimation? If someone knows,...
07 January 2023 5,147 2 View
We want to estimate NOR activity in Pseudomonas aeruginosa. Can anyone share a protocol for the same? A protocol based on UV-vis spectrophotometry would be more likely to be executed in our lab...
23 May 2022 6,017 0 View
The genus Prevotella comprises of anaerobic bacteria. What I want to know is whether they are strict anaerobes, or can tolerate oxygen. Is it necessary to have a atmosphere rich in CO2 to...
16 September 2016 6,537 1 View
Sometimes, while screening natural products for antimicrobial / anti-infective potential, the results do not show any pattern; i.e. with increase in concentration either there is no increase in...
12 February 2016 7,868 2 View
Pyoverdine is a known siderophore. What about other pigments, like prodigiosin, pyocyanin, staphyloxanthin, violacein, etc.? Can they chelate iron?
12 February 2016 8,363 1 View
Can higher pigment production in bacteria (e.g. Serratia marcescens, Staphylococcus aureus, Pseudomonas aeruginosa, etc.) in presence of a particular compound(s), be taken as an indication that...
11 February 2016 9,141 2 View
Currently there has been a considerable interest in screening natural products for their potential quorum-sensing inhibitory potential. Though these inhibitors can be effective therapeutic agents,...
04 August 2015 211 4 View
We are looking for a simple method which does not require any sophisticated instrumentation. I think there is some method wherein some substance of known molecular weight can be added into the...
11 July 2014 9,463 3 View
Surfaces like glass can be tried, but it is usually convenient to work in multi-well plates, so I would like to know whether any modification in media etc. can help. Addition of sucrose, we have...
27 June 2014 9,157 3 View
One S. mutans culture with us was forming good biofilm until recently. But since last few experiments, its biofilm formation is not satisfactory.
27 June 2014 7,064 4 View