After running agarose gel electrophoresis I have observed a smear on the agarose gel. Does the smear indicate that I have isolated DNA properly?
what is your method in DNA isolation ? the DNA should appear like one big band
Hi,
It depends on the type of DNA you isolated. If it's a plasmidic DNA you should see the 3 DNA isoforms ( resulting in 3-4 bands of different intensity). If its a PCR or is a plasmid digestion you should see only the PCR band or the bands corresponding to the fragment expected from de digestion.
If you see a diffused smir probably there is a degradation of your DNA ( check for contaminants) or simply repeat the gel.
Anyway if you post the gel picture with more information about the DNA sample, we could help you more =)
A smear indicates that you have different sized DNA fragments running down your gel (bigger fragments closer to the origin). If you have only amplified one gene you should only see one band per lane (or per sample).
@Nicky: Without a PCR he couldn't amplified one gene! He only made a DNA extraction! Cheers, Nadine
@ Assma Almohaidi I have isolated metagenomics DNA from soil with the help of commercially avaliable kit.
did you made Nanospec for your sample before PCR to know the concentration and purify of your sample
or you Loaded very little amount from PCR product or much amount
try to made nanospec for your DNA extraction sample to be sure your kit work or not
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