Protein carbonyls are important compounds to measure progression of oxidative stress in tissues.
Available articles online do not have details about how to do final estimation/calculation of the carbonyl content.
You may consult the following papers to develop your protocol:
Article Direct Characterization of Protein Adducts of the Lipid Pero...
Article Oxidative damage to proteins: Spectrophotometric method for ...
Thanks Iqra, your suggestions are on point. However, I can't access the full articles.
I will be glad if you can help download and send to me as attachments
Israel Ebhohimen please share your email address.
@Iqra Zaheer
[email protected]
The factor 28.2 is used in the calculation based on oleic acid. Will this factor change for shorter fatty acids?
03 April 2019 9,827 3 View
Structure Related Activity and other necessary data that can now be easily obtained can easily help in the synthesis of leads
06 July 2018 1,184 3 View
It is a whole lot much easier to find a solution when the problem is well understood. Available in silico tools afford scientists today this unique approach of first understanding the biochemistry...
01 January 1970 3,628 4 View
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Hi all! My thesis groupmates and I are working on purifying an E. coli bacteriophage. The phage was handed down to us by the previous thesis group, but they only managed to purify it to about...
06 August 2024 5,801 4 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
What is the role of oxidative stress in toxin-induced carcinogenesis? I am currently researching the impact of environmental toxins on children's health and would greatly appreciate insights from...
02 August 2024 3,174 1 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
i compare between the antioxidant and oxidative stress level between negative control rats (saline) and CCL4 intoxicated rats. i found the level of GSH and TAC is more in toxic group than normal....
26 July 2024 1,887 0 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View