Why I don't get results from Restriction enzyme analysis by using iso-schizomers of restriction enzymes mentioned in the publication?

I am using Psy I (is-schizomer of Asp I) and my PCR product didn't get in digested form. Instead I see the only band on the gel is that of my PCR product (i.e 900b.p). I use enzymes from...

02 February 2020 2,130 2 View