We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the pixelated background is also identified as spots in the software. I am just wondering what are the criteria's/settings that we should provide in the Biorad imaging station (Chemi Doc XRS) while taking the images for 2D analyses to avoid the problem of pixelation. Please provide me with suggestions.
Thanks in advace,
MDV
Cropping the gel always decreases the dpi as it increases the size of gel. I suggest you to check the “dpi” at which you have scanned your gel. The prob u are facing happens when you scan the 2D gel at lower dpi. Scan your gel at 300dpi and I feel that you will not face this prob again. Don’t scan it at higher dpi as PDQuest will not accept too large image.
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