Muralidharan Vanuopadath

4 Questions 15 Answers 0 Followers

Questions related from Muralidharan Vanuopadath

Why can't I see any protein spots after a 2D run?

I run 10% SDS-PAGE gels, at 100V for 2 hours. When I did a 1D SDS gel there were lots of protein bands. However, when I use IPG strips (pH3-10), do IEF overnight and run the gels, no spots...

19 November 2014 5,039 16 View

Why are proteins not fully soluble in the buffer after methanol-chloroform precipitation?

We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...

06 November 2014 6,003 4 View

Can anyone help with 2D gel images seems to be pixelated while cropping the images in PD Quest software?

We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the...

22 August 2014 9,154 2 View

Does anyone know the components for preparing complete RPMI 1640 media for culturing THP-1 cells?

The cells are procured from ATCC and they recommend to use the specified media supplied by ATCC itself. But since sigma is also providing the same RPMI 1640 media, I thought of going with that....

24 February 2014 8,019 5 View