8 Questions 31 Answers 0 Followers
Questions related from Muralidharan Vanuopadath
After 2D I got an image like this with streaking in the gel and seems like the proteins are poorly resolved. This is the image obtained after silver staining. Can anyone help me in getting this...
24 November 2014 3,656 10 View
I run 10% SDS-PAGE gels, at 100V for 2 hours. When I did a 1D SDS gel there were lots of protein bands. However, when I use IPG strips (pH3-10), do IEF overnight and run the gels, no spots...
19 November 2014 5,094 16 View
Why is the desired set voltage not reached or reached very slowly while focusing? The voltage is increasing but not in a manner as it was working before. The maximum current limit is reaching (50...
08 November 2014 920 13 View
We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...
06 November 2014 6,112 4 View
We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the...
22 August 2014 9,247 2 View
We have done the analysis of a standard compound both in positive ionization (PI) and negative ionization (NI) mode with varying concentrations (0.01nanomol-10nanomol). But it seems that the ion...
18 July 2014 706 5 View
I just want to know what are the parameters are that we have to set in an IEF cell for the focussing of a 7cm IPG strip. Initially we followed the guidelines as provided in the instruction manual...
05 April 2014 1,179 3 View
The cells are procured from ATCC and they recommend to use the specified media supplied by ATCC itself. But since sigma is also providing the same RPMI 1640 media, I thought of going with that....
24 February 2014 8,111 5 View
