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Questions related from Muralidharan Vanuopadath
I run 10% SDS-PAGE gels, at 100V for 2 hours. When I did a 1D SDS gel there were lots of protein bands. However, when I use IPG strips (pH3-10), do IEF overnight and run the gels, no spots...
19 November 2014 5,051 16 View
We precipitated the proteins using methanol-chloroform protocol and redissolved the obtained protein pellet in Tris/PBS buffers. But the proteins are not completely dissolved leaving behind a mass...
06 November 2014 6,029 4 View
We have taken the gel images of a 2D run and was trying to analyze those images in the PD Quest Basic software from Biorad. But the images are not clear when we are cropping it and seems like the...
22 August 2014 9,177 2 View
The cells are procured from ATCC and they recommend to use the specified media supplied by ATCC itself. But since sigma is also providing the same RPMI 1640 media, I thought of going with that....
24 February 2014 8,039 5 View