Can we use glass sleeve as an alternative for quartz sleeve?

I am planning to design a lab scale UV reactor for sulphate radical based advanced oxidation of paper mill effluent. Can I go for glass sleeve instead of quartz sleeve for the UV lamp ? Can anyone...

24 January 2019 8,511 3 View

What all thing we should keep in mind while selecting a solvent for hydrothermal reaction in a 50 ml autoclave?

I am going to use a 50 ml teflon lined autoclave for doing hydrothermal synthesis and I am deeply concerned about its safety. The pressure limit inside the autoclave is 3MPa and temperature...

14 February 2018 7,313 3 View

What is the reason for big white pellet problem in plasmid isolation?

In plasmid DNA isolation by alkali lysis method, I get big white pellets after isopropanol washing. It is not dissolving and it also not resolving in gels afterwards. Please any suggestions.

17 December 2017 8,213 2 View

Are there any distinct morphological characters by which Anopheles subpictus species B can be distinguished from other sibling speices?

The available morphological character for distinguishing adults of species B is the lengths of apical white band and subapical dark band of female palpi. However, there are reports of variations...

25 January 2017 1,400 4 View

Is it possible to isolate/detect Japanese Encephalitis virus from Bird droppings?

Migratory birds are likely to transport JE viruses from an endemic area to a non-endemic area. Screening their blood would be the best method to detect JE virus in them. However, it is not...

01 April 2015 8,156 12 View

How can I add manually digitized drainage networks in the ArcSWAT model?

I have created drainage network automatically using ArcSWAT model and DEM with

05 May 2014 3,401 3 View

What is the current status of the Zika virus in Asia?

26 February 2014 7,707 3 View

Does anybody know various theories on the origin and evolution of insects?

In our entomology syllabus one of the components is origin and evolution of insects-various theories. Can anybody suggest a suitable book/paper dealing with this issue?

25 November 2013 4,229 3 View

Can anybody identify the species?

It was found inside an unopened packet of Killer Zone trilogy CD for PS3 brought from the US.

22 November 2013 9,553 14 View

T-test as a statistic for the analysis of fMRI data - can anyone help?

Why are t-tests preferred over other statistical tests for the analysis of fMRI data?

05 October 2013 8,594 8 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Does any one know how i can prepare liver tissue with transmission electron microscope with good fixation?

i have problem in preparation of liver tissue with bad cell organelles and how i can adjust osmolarity

03 March 2021 8,890 1 View

No title

02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

Interested to stain mice tissue with WGA 488 conjugate, anybody has a protocol to recommend?

Interested to stain mice brain tissue with WGA 488 conjugate, appreciate if anyone willing to offer me some guidance or suggestions! Thank you!

28 February 2021 3,951 2 View