What should be the protocol for finding MMP1 from collagen type 1 zymography?

01 February 2017 1,831 3 View

How to extract DNA coated on whatman FTA classic cards?

I tried all the methods available on internet be it methanol extraction or using Qiagen kit. I also tried incubating FTA card pieces in TE buffer overnight at 56 C but i got nothing. Each spot is...

11 December 2016 6,031 4 View

What will happen if i use dream taq polymerase instead of taq polymerase im my PCr reaction?

Taq polymerase i used was 1u/uL, Dream taq was 5u/uL. I wanted 1.25u so i took 0.25uL of Dream taq and my pcr product showed smeared images.

11 December 2016 4,407 16 View

Can RAW 264.7 cells remain constantly activated in culture due to low amount of endotoxins present in diluted 10X PBS with autoclaved distilled water?

02 March 2016 6,643 5 View

What are the similarities and differences between tumor associated antigens and self antigens?

31 December 2014 8,344 4 View

Can vaccines be targeted towards non-structural proteins and how effective it would be in comparison to structural proteins?

whether structural proteins are good for vaccine development or non-structural proteins?

31 December 2014 8,106 5 View

Can anyone explain possible reasons for anti cancer activity of drug at lower concentration?

My drug shows anti-cancer activity at as low as 5ug/ml whereas literature available shows activity around 200 to 1000ug/ml. Any possible reasons? I am doing MTT to test drug efficacy of my drug....

01 January 1970 5,766 1 View

How can I measure MMP-1 protein activity or protein level from liver tissue?

Instead of checking only the mRNA level, I want to check the active protein level of MMP-1 in Liver tissue from mice. How can I do that?

03 March 2021 1,763 2 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

IHC on mouse liver hepatocytes?

Can anyone help me choose primary antibodies for mouse liver hepatocytes and also in conjunction Cd8 cells. Also Cd3 cells. I want to look at hepatocytes and adjacent immune cells in mice with...

01 March 2021 6,953 1 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

How to avoid ulcers in s.c. injected tumors ?

Recently, we have injected s.c. B16F10 tumor cells in BL/6 mice. At around 12-15th day some animals showed ulcerations in tumors , with a big hole in the middle (it looks like a volcano). We have...

01 March 2021 9,938 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View