Especially in India
Try this web: http://ninindia.org/lab_animals.htm
Currently i am following this protocol: 1mg/ml collagen, 0.5 mg/ml collagen in 10% resolving gel. 2x15 mins; 2.5% triton X-100 wash incubation buffer (50mM tris, pH-7.4, 10mM cacl2, 0.05% Brij...
01 February 2017 1,903 3 View
I tried all the methods available on internet be it methanol extraction or using Qiagen kit. I also tried incubating FTA card pieces in TE buffer overnight at 56 C but i got nothing. Each spot is...
11 December 2016 6,101 4 View
Taq polymerase i used was 1u/uL, Dream taq was 5u/uL. I wanted 1.25u so i took 0.25uL of Dream taq and my pcr product showed smeared images.
11 December 2016 4,486 16 View
Without any activation, I constantly observe activated RAW 264.7 cells in culture confirmed through cytokine assay of TNF. Even after a number of passages (Passage number 25 to 40) are given, TNF...
02 March 2016 6,714 5 View
Tumor associated antigens are self antigens, hence how are they different from tumor associated antigens?
31 December 2014 8,473 4 View
whether structural proteins are good for vaccine development or non-structural proteins?
31 December 2014 8,197 5 View
My drug shows anti-cancer activity at as low as 5ug/ml whereas literature available shows activity around 200 to 1000ug/ml. Any possible reasons? I am doing MTT to test drug efficacy of my drug....
01 January 1970 5,831 1 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
Can anyone share any reference that gives mouse specific CD3 antibody sequence. I have been searching for this since few days and no luck. Thank you very much
08 August 2024 9,157 4 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
i have sorted anti-NP specific plasma cells from bone marrow of C57BL/6 mice at certain times after immunization with variable counts and isolated total RNA using TRIZOL method for RT-PCR using...
05 August 2024 8,835 1 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
