Sanjai kumar Pillala

8 Questions 12 Answers 0 Followers

Questions related from Sanjai kumar Pillala

What should be the protocol for finding MMP1 from collagen type 1 zymography?

Currently i am following this protocol: 1mg/ml collagen, 0.5 mg/ml collagen in 10% resolving gel. 2x15 mins; 2.5% triton X-100 wash incubation buffer (50mM tris, pH-7.4, 10mM cacl2, 0.05% Brij...

02 February 2017 1,873 3 View

What will happen if i use dream taq polymerase instead of taq polymerase im my PCr reaction?

Taq polymerase i used was 1u/uL, Dream taq was 5u/uL. I wanted 1.25u so i took 0.25uL of Dream taq and my pcr product showed smeared images.

12 December 2016 4,452 16 View

How to extract DNA coated on whatman FTA classic cards?

I tried all the methods available on internet be it methanol extraction or using Qiagen kit. I also tried incubating FTA card pieces in TE buffer overnight at 56 C but i got nothing. Each spot is...

12 December 2016 6,066 4 View

Can RAW 264.7 cells remain constantly activated in culture due to low amount of endotoxins present in diluted 10X PBS with autoclaved distilled water?

Without any activation, I constantly observe activated RAW 264.7 cells in culture confirmed through cytokine assay of TNF. Even after a number of passages (Passage number 25 to 40) are given, TNF...

03 March 2016 6,685 5 View

Can anyone help me to find trpv knockout mice or trpv knockout cell line available?

Especially in India

05 May 2015 8,096 1 View

Can vaccines be targeted towards non-structural proteins and how effective it would be in comparison to structural proteins?

whether structural proteins are good for vaccine development or non-structural proteins?

01 January 2015 8,158 5 View

What are the similarities and differences between tumor associated antigens and self antigens?

Tumor associated antigens are self antigens, hence how are they different from tumor associated antigens?

01 January 2015 8,431 4 View

Can anyone explain possible reasons for anti cancer activity of drug at lower concentration?

My drug shows anti-cancer activity at as low as 5ug/ml whereas literature available shows activity around 200 to 1000ug/ml. Any possible reasons? I am doing MTT to test drug efficacy of my drug....

01 January 1970 5,806 1 View