Difficult to figure out the concentration range for customizes oligos?

Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...

31 December 2017 5,310 7 View

Difficult to figure out the concentration range for customizes oligos?

Dear Researchers, I have purchased my designed oligos from Sigma for miR based silencing studies as sense (~64nts) and antisense (~64nts); together if annealed it is ~64bp. I take equimolar...

31 December 2017 247 5 View

How to overcome the issues in cloning of amiRNA(with miR155 backbone into mammalian vector(pcDNA3.1-eSIBR(intronic region containing BIC region)-GFP)?

Dear Researchers, I have custom designed mammalian vector pcDNA3.1 containing the eSIBR region that has the BIC sequence and GFP downstream to the CMV promoter. The eSIBR region has a polylinker...

10 November 2017 9,631 0 View

Dear Researchers, Could anyone suggest me to overcome the troubles in cloning siRNA with an miRNA backbone into mammalian vector?

A siRNA is replaced in the sense and anti sense regions of an Mirna without disturbing it's backbone and is placed in a synthetic intronic region. I have the intronic region with a poly linker...

09 October 2017 9,627 0 View

Why and how DNA degrades after elution?

Hello Researchers, I am trying to clone my target genes into a promoter region of a vector (pcDNA3.1-GFP). I use 1 ug/ml of vector for restriction (20 ul volume) and I follow Invitrogen purelink...

03 April 2017 1,926 6 View

Can miRs from different chromosomal loci interact with each other?

Dear Researchers, Its well known that miRs after their complete biogenesis are released into the cytoplasm and they involve in different functions. I do have read from literature that certain miR...

02 March 2016 6,650 6 View

What are the possibilities of fungal contamination apart from handling?

08 September 2015 2,807 12 View

Do the plant extracts influence the staining intensity?

I use plant extracts of varying concentrations from 1 ng to 10 ug for estimating lipids in 9 day old 3T3-L1 adipocytes by Oil Red O staining. The cells are treated with the extracts throughout the...

06 July 2015 5,028 8 View

Does copper sulphate influence the growth of 3T3-L1 cells incubated in CO2 incubator?

3T3-L1 cells grow healthily when the humidity of the incubator is maintained by using a tray of water but it grows unhealthily and slow when the copper sulphate solution is used to maintain...

01 February 2015 8,903 4 View

Is it acceptable to get a percentage value higher than 100 for my glucose uptake assay in 3T3-L1 cells?

3T3-L1 cells are insulin sensitive but when they are counted and seeded for insulin stimulated glucose uptake assay, it results with 123% of glucose being up-taken by the positive control cells...

31 December 2014 828 7 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

What do you know about biotechnology art?

What do you know about biotechnology art?

02 March 2021 1,653 3 View

Which is the best place in the world for the plant biotechnology research?

I am searching for a good place for the Post Doc,

02 March 2021 4,053 3 View

What SPSS analysis should I run with one IV (with two levels) and two DVs?

Hi, I am trying to figure out which test would be most appropriate to run for the following question: Is there a difference in task blood pressure between smokers and non-smokers? There are two...

01 March 2021 8,235 2 View

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

Could you please recommend some good journal that accept very long revie papers (approximately 6000+ words) in neurosurgery, cancer biology ?

01 March 2021 8,087 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Which bacteria are suitable for quorum sensing screening?

I want to find a quorum sensing inhibitor from plant extracts. Klebsiella pneumoniae, Moraxella catarrhalis, Staphylococcus aureus, and Streptococcus pneumoniae. I looked for these strains...

01 March 2021 7,675 3 View

Why might my GFP tag be dissociating from my plasmid after transfection?

I have a set of stably transfected cell lines all transfected with plasmids containing GFP tags on the C terminus. During a western blot using anti-GFP antibody, one of my plasmids has dissociated...

01 March 2021 9,310 4 View

Isolates of Hymenoscyphus fraxineus?

Dear Dr. Cai, my name is Simone Prospero and I work in the team of Phytopathology at the Swiss Federal Institute for Forest Snow and Landscape Research (WSL;...

01 March 2021 1,133 1 View