How to improve the adhesion of epoxy coating on steel substrate? Which factor affects?

I am facing a problem regarding a de-adhesion problem with the coating. A brief description of my methodology is given below. I am using mild or Low Carbon steel as a substrate and I polish the...

14 February 2021 2,167 4 View

Can anyone help me with the problem of hyperelastic coefficients in abaqus?

Hello Everyone, I have done a uniaxial test on rubber and data (attached with the post) has been used in Abaqus to get YEOH's coefficient. But I am getting a warning that it is unstable? Can...

04 February 2021 5,975 3 View

Can you please help with the problem modeling a damper in ABAQUS?

Hello everyone, I am trying to model a damper in ABAQUS. I have obtained hyperelastic properties using a uniaxial tensile test and viscoelastic properties by relaxation test conducted in the...

09 January 2021 4,515 1 View

Separation of protein via Akta system gave broad peak during elution. I wonder is it normal to obtain broad peak ?

Greetings.. I am using Akta Pure system for my protein purification. I have optimized buffer system for protein elution. However, this time I got broad peak while eluting sample. For...

24 December 2020 393 7 View

Is there any relationship/correaltion between 1) elastic modulus and adhesion or 2) elastic modulus and density of solid material or polymers?

21 December 2020 1,853 3 View

Can anyone help me with the constraint between two rods?

Hello Everyone, I am trying to model a wire mesh in abaqus. I have modeled it with 1mm circular cylinder. I want to give a tie constraint to the perpendicular wires. I am unable to find it in...

16 December 2020 3,572 6 View

How to convert Time Domain DMA data to prony series?

Hello Everyone, I have Time Domain DMA data of rubber i.e. loss modulus and storage modulus varying with time with constant 1 hz frequency. I need to convert it into prony coefficient. I need to...

09 December 2020 5,211 3 View

Hi can you tell me how may I calculate the selectivity and relative selectivity coefficients of the Surface plasmon resonance sensor?

This is related to my research

15 November 2020 8,455 3 View

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. What it could be?

While running 15% SDS gel, I have noticed some blurred blue coloration above sample protein. I have attached figure for the convenience. Suggestion and answers in this regard will be highly...

28 October 2020 2,869 1 View

Single Stage deep learning based instance segmentation?

Hi, Is there any single-stage deep learning based instance segmentation method that outputs single channel mask where each pixel indicates instance labels (assume we are considering one class:...

21 September 2020 9,211 4 View

Looking for Materials with high thermal expansion coefficient and high temperature resistance?

03 March 2021 8,272 1 View

How do I increase the yield of my PCR product?

Hello, We would like to increase the yield of our PCR product. We are running a series of PCR reactions that is targeting ~1.1kb sequence. We begin each reaction with ~400pg of template DNA...

02 March 2021 4,029 3 View

Research on Supply Chain Management in Indian context?

Hi, I am planning to apply for the PhD degree in the Supply Chain Mgt. with specific area of "Cold Storage warehouses" during Pandemics and wars. Where lock downs and shut downs are frequent....

02 March 2021 285 2 View

PACYC PCR not working after several attempts. Would anyone have any suggestions?

So, I have been trying to run a pACYC PCR which will be used later on for a Gibson Assembly. However the PCR is not working. I have already tried gradient PCR and changing extension time; however...

02 March 2021 1,146 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

How to address this issue in the analysis of Real-Time PCR results?

To dear Researchers, I was analyzing a series of concentration for estimation of Real-Time PCR efficiency. The concentration was 1:10. I used MS-excel to evaluate Slope. The result of slope was -8...

01 March 2021 8,683 4 View

No title

Does anyone have the experience of using Taq Man probes in the QIAGEN Rotar- Gene qPCR machine?

01 March 2021 5,311 1 View

MiRNA qpcr problem?

Dear All, mirna primer showing some problem in the melting curve? any idea why? As attached is the melting curve. The forward sequence is obtained from miRBase and reverse primer is universal.

28 February 2021 5,008 4 View

Runs of nucleotides in Sanger sequencing?

I performed site directed mutagenesis, transformation, and then I sent out plasmids for Sanger sequencing and found out that there is extension of DNA just before the stop codon. I am not sure...

27 February 2021 547 3 View

Can thermal cycler machines be problematic during PCR?

Hi, my question is about the heating of thermal cycler machines and I hope some of you had experienced a similar thing previously. There are two thermal cycler machines in the lab(BioRad) and for...

26 February 2021 4,777 4 View