I isolated a fungus from plant tissue and am having trouble identifying it. I grew the fungus on PDA and made a slide culture which was stained with lactophenol cotton blue.
If it doesn't make any spores it may be Rhizoctonia but must confirmed by molecular
I am currently working on LncRNA; to know the lncRNA-protein interactions I want to do RNA pull down assay, so I need to design primers with T7 promoter. I need assistance in this regard.
07 August 2024 6,622 1 View
Silver Nanoparticles docking with alpha amylase and alpha glusidase .
11 July 2024 6,883 2 View
Yesterday, my Agros gel broke by itself (unknown reason). I was busy & gel remained on the caster for at least one hour.
04 June 2024 5,063 0 View
The question asks about the timeframe for running gel electrophoresis after nuclease-free water has been added to DNA samples.
03 June 2024 1,639 3 View
I am working on looped configuration, TAE1, Alternator TAE2 and TAR I want to know the best configuration with maximum efficiency Note: The alternator must be NASA's linear alternator, not the...
08 May 2024 7,922 0 View
Hi respected colleagues! While trying to form a second union of domains components in a 3D COMSOL model, I was met with the the response "Error while building 'Union 2' in Geometry: Boolean...
07 April 2024 863 5 View
Dear LinkedIn Family, Greetings of the day from Pakistan. I am Actively seeking for Collaboration in Research, Training programs students/faculty Exchange program globally. Area of Interest...
03 April 2024 3,312 1 View
Explain the method of writing literature review in my thesis?
31 March 2024 3,013 1 View
The process of development of quasispecie in HCV.
11 March 2024 9,201 1 View
I need more clarity on the question.
16 February 2024 5,308 2 View
I'm trying to find a DNA extraction method for fungi that does not require equipment and heating. Is there anyone who can suggest an alternative option? Thank you
08 August 2024 4,733 2 View
I am staining some brain sections stored in cryoprotectant that express a Histone H2B- GFP fusion protein that were generated ~10 years ago. I know I need to enhance signal with an anti-GFP...
07 August 2024 5,338 2 View
Hello everyone, I have recently started using the microtome device for sectioning of paraffin-embedded mice lung. While I had some success in sectioning and observing proper ribbons, some of my...
06 August 2024 666 4 View
To compare positive and negative cell populations in flow cytometry, should I compare unstained cells with antibody stained cells? Or with the isotype control? Most papers show comparison with...
06 August 2024 6,728 6 View
Hi all, I was just wondering if anyone has experience with multiplexing a mouse monoclonal primary and a rat primary. I'm trying to multiplex by incubating them in the same well but was told by a...
06 August 2024 9,710 1 View
I will be with my students collecting seaweed samples in a marine farm and later we will process this tissue for RNA isolation and further sequencing. Does anyone have tips on how to collect the...
04 August 2024 501 2 View
I heard an explanation about something being a better proton acceptor or lone pair donor but that doesn't make sense. I couldn't explain in in terms of acid-base theory. The hand-waving way I saw...
03 August 2024 9,918 0 View
Hi guys If anyone is currently working on aging cells, you guys would like to give me some advice. I'm testing against biomarker (SA-beta-Gal), I encountered a false positive in the control group...
02 August 2024 6,735 1 View
Now research fellow used it to prevent fungus in potato,may be potatoes gene effect.
02 August 2024 180 3 View
All plants are green but some of these plants becomes yellow. I did not found any reason. Please help me to find out the real problem.
01 August 2024 589 4 View