I am planning to perform MTT assay for determining the anti-apoptotic activity of MSC derived exosomes on drug treated neurons. Following are my doubts.
1)to collect exosomes should I allow the MSC's to reach complete confluency in culture flasks, keep them in that state for 48 hrs and then collect the conditioned media.?
2) what dilution range of exosomes (ug/ml) should I use for adding to the 96 well plate or what should be the highest dilution i should start with ?
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definitely jan , thanks a lot for replying . It is unexpected but at the same time encouraging.
Personally, I would perform a serial dilution with exosomes (within normal physiological ranges), then I would also perform a time titration at each dilution. Then add the MTT.
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