Do you know the excellent FAO manual?

http://www.fao.org/docrep/004/t0117e/t0117e00.htm

If you need more specific information, let us know.

Never forget that bovines, by nature, are MONOVULAR! It is impossible to succsessfully superovulate bovines without ruling out the inherent (naturally evolved) physiological dynamics of ovarian follicular growth.

Before starting to deal with MOET, try to understand the importance of physiological ovarian function, and always keep in view detrimental aftereffects of reproduction techniques that fundamentally disregard physiology.

When it comes to evaluating modern bovine reproduction methods, bear in mind the difference between 'clever' and 'wise'!

HI,

Dear Mr Adewuyi Abdulmujeeb,

I have so information about this, but i have a valid source to you., He is one of the best distinguished professor in this area, " Dr M. Kafi from Shiraz university, Shiraz, Iran.

His address is:

https://www.researchgate.net/profile/Mojtaba_Kafi

you can qsk your question and also tell him, Dr Ommati introduced me.

Good Luck.

M.M.Ommati

 Superovulation is a very inefficient method of obtaining oocytes from bovine ovaries and is likely to be replaced by other approaches within the next decade. However, superovulation results in about ten times more embryos than single ovum recovery. Without superovulation, a usable embryo can be recovered about 60 percent of the time from normal donors by skilled technicians. Under similar conditions, superovulation usually yields an average of six usable embryos, although the variation is astounding (Figure 1). Normally, no embryos are recovered from 20–30 percent of superovulated donors and only one to three embryos are obtained from another 20–30 percent (see Table 11). An ideal response of five to 12 embryos is obtained from about one-third of the donors. However, a small percentage of donors yield more than 20 good embryos and, very rarely, more than 50.

The two generally accepted methods of superovulating cattle are based on two different gonadotrophins, although there are many minor variations of these methods. The simplest is to give an intramuscular (i.m.) injection of 1 800–3 000 IU (usually 2 000–2 500 IU) of pregnant mare's serum gonadotrophin (PMSG), more correctly designated equine chorionic gonadotrophin (eCG), followed by a luteolytic dose of prostaglandin F2 alpha or an analogue i.m. two to three days later. A second prostaglandin injection is often given 12–24 hours after the first, and seems to improve embryo production.

The second method of superovulation is to give eight to ten injections of follicle stimulating hormone (FSH) subcutaneously (s.c.) or i.m. at half-day intervals. Intramuscular injection is more reliable under field conditions. As with PMSG, prostaglandin F2 alpha is given 48–72 hours after initiation of treatment with the fifth, sixth, or seventh FSH injection. The most common FSH regimen is 6,6,4,4,2,2,2, and 2 mg at half-day intervals with prostaglandin F2 alpha given with the sixth or seventh FSH injection. About 20 percent more gonadotrophin should be given to cows weighing over 800 kg. Sometimes, higher doses are used for the first two days; others give 5 mg for each injection. There are few studies with adequate numbers of donors per treatment group in which constant and decreasing doses have been compared, so reliable conclusions cannot be drawn regarding efficacy of such regimens.

Mohammad Mehdi Ommat, you obviously copied your information from the FAO document for which I provided the link above.

I don't want to start an argument here, but the remarks about the efficiency of superovulation in cattle are opinions, at best.

The FAO document is dated of 1991, i.e. 24 years ago, and MOET still is the most reliable and efficient method to produce multiple offsprings from a donor.

20-30% of non-responsive donors is indeed not very efficient, but does not reflect the result of competent specialists, which are rather in the 5-10% range, provided fertile semen is used and donors are selected accordingly.

The average of useable embryos per flush vary greatly among sources and practitioners, generally they range between 4.5 and 11(in average!).

You may want to have a look at the national statistics of the AETE for this purpose, page 11 of the proceedings linked below.

http://www.aete.eu/pdf_publication/42.pdf