There is no gauranty that your gene in wheat would have the same sequence as that of Arabidopsis. It may have copy number or structural variations. If BLAST cannot help you. Then only sequence using random primer will help you.

You may check functional analysis of candidate gene if published.

Does the Gene in Arabidopsis, rice, maize have established orthologs in sorghum??? If yes, that could be a good starting point. Many genes in these three plants could be point to wheat orthologs in their respective databases. When you blast, what criteria to you use to decide if a hit is good enough to be considered as an ortholog?

Hi,

So keep in mind that the sequence homology is not necessarily very high if it is not a structural gene or a very conserved pathway. If you have name of the gene or a signature motif (specific domain) you could try to look for that. Phytozome is a pretty good option because you can select the species you are looking into. Most search engines automatically exclude homologs with low level of similarity if there are such with high.

https://phytozome.jgi.doe.gov/pz/portal.html

This is the website and then you can select tools -> blast or key word -> select species. You can use complete or partial sequence. You can also try to look for the protein sequence directly.

Good luck!

dear you should find the gene id of that gene in arabidopsis from the published article then using that gene id find the sequence from NCBI and blast it and you will find the smiller sequence in wheat genome from NCBI

Yes, the website: https://phytozome.jgi.doe.gov/pz/portal.html

could be a good start.

Hi. I hope the following article might help:

https://www.hindawi.com/journals/ijpg/2015/874361/

Thank you, everyone, for your precious suggestions.

Zeeshan Ghulam Nabi Gishkori Nice answer