We are trying to measure cellulose and hemicellulose content in soil by acid hydrolysis using sulfuric acid. But we are not getting significant peaks in HPLC. Another problem we are facing while determining lignin through loss on ignition procedure. Please suggest/refer to a suitable protocol to overcome this problem.
Maybe you are right. The concentration might be low in soil samples, but we don't have any idea about that. Can you cite the reference or protocol for concentrating sugar in the sample?
You can concentrate the samples with Nitrogen gas. You can use block sample concentrator or evaporators for this.
Try this:
H. Yang, R. Yan, H. Chen, C. Zheng, D. H. Lee, and D. T. Liang, “In-Depth Investigation of Biomass Pyrolysis Based on Three Major Components: Hemicellulose, Cellulose and Lignin,” Energy Fuels, vol. 20, no. 1, pp. 388–393, Jan. 2006, doi: 10.1021/ef0580117.
Although this is mainly for biomass, you can still give it a shot.
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