Hi all!
Our lab is using the vector elite ABC kit for IHC in brain tissue. We go through a ton of these reagents, and as we all well know, they are expensive.
I'm wondering if anyone has suggestions for alternatives (more reagent for less money), or (even better) a protocol to make their own. I'm only using the AB reagents, all of our other antibodies are purchased separately.
I've looked up standard IHC protocols for DAB staining in some of my old books, but the protocols are pretty unclear and I'm hoping someone might have a tried and true method and which reagents they use.
Thank you all for your continued help and support!
Tanya
You might take a look at Rockland's IHC kits:
https://rockland-inc.com/AdvancedSearch.aspx?SearchText=immunohistochemistry+Kits+&category=Kits&p=1&ddlSampleSize=&subcategory=
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