How can I go about in silico studies using chemical compound identified in fraction from column chromatography using HPLC or GCM-M?

I want see if there is interaction with compounds characterised with either HPLC or GCM-M with any proteins in silico

31 December 2018 8,459 3 View

Can one remove slovent from nonpolar solvent extract through Lyophilization ?

lyophilizer can remove non polar solvent from extract just as with water

08 September 2018 7,029 2 View

Can i get a youtube link or any material on the use of graph pad prism ?

most statistical analysis or graph in my study are done using SPSS but i want to explore the use of graph pad prism.

04 May 2017 3,241 1 View

Can I get materials, jornal papers or links that can help in learning and mastering the use of PyMOL ?

I need the the latest application of the software application for the antimalarial principle identification in my study. I need more enlightenment on the usefulness in toxicological studies

04 May 2017 8,089 1 View

How can one synthesize ZnO naonoparticle using ZnO salt which is insoluble in water and aqueous laef extract?

The experiment is green synthesis of ZnO nanoparticle using aqueous extract.

08 September 2016 1,526 3 View

What is the ratio of plant extract to salt in green synthesis of nanomaterials?

this is to know the proportion of the extract that should be added to the salt to use for green synthesis

10 November 2015 3,504 4 View

How effective is calcination in nanomaterial green synthesis and which other methods could be preferred?

10 November 2015 9,259 7 View

Which statistical package should be preferred for more accurate result for biological experiments?

best statistical package for statistical analysis

06 July 2015 8,340 0 View

Can anyone send to me any publication or book on chemoinformatics and bioinformatic?

this to enhance my knowledge on this concepts

04 May 2015 5,114 0 View

Can one identify an unculturable microorganism using meta-genomic technique?

a fermentation of our local seed were fermented and we have some suspicious that certain other micro-organism could be responsible and want see if meta-genomic technique be useful in confirming it

04 May 2015 1,916 1 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

No title

02 March 2021 3,060 3 View

New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before?

Question to you and THEM, the New Journal, "Integrative Psychological and Behavioral Science" -- do you not know, and have you not seen, this done before? There appears to be a core problem for...

02 March 2021 3,024 2 View

Does anyone know HRM channel in a 2 plex QIAGEN Rotor- Gene Q qPCR can be used as a separate channel?

I am going to have 3 different probes in my qPCR work that I am going to do. But I realized that the machine we have in the lab is a Rotor-Gene Q 2plex HRM Platform, saying it has green, yellow,...

01 March 2021 8,544 1 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View

Is CD44 degraded in lysosome together with hyaluronan after CD44 mediated endocytosis of hyaluronic acid?

Also when RHAMM binds hyaluronic acid, they get internalized, will RHAMM also be degraded? Or both CD44 and RHAMM will be transferred back to the cell membrane? Asking for breast cancer cell line...

01 March 2021 8,169 2 View

Do you need the CMV enhancer before the CMV promotor for a proper function of CMV on the gene that follows?

I'm a student and I have to produce a cell line with a knockin for the NRF2 gene with GFP. I have to put a promotor in front of the GFP because the gene will be too far away from the promotor of...

28 February 2021 7,127 2 View

What could be some reasons why unstimulated monocytes are getting activated?

Im doing PBMC isolation -> CD14+ enrichment using magnetic beads -> stimulation setup. My negative control is just cells in cRPMI but they seem to get activated over and over again.

28 February 2021 7,883 3 View