Expert in Diabetes research and in vitro culture from pancreas
Is there a free software to perform simulations and to deconvolute mixtures of compounds in FORS and Raman data? I would like to: - simulate mixtures of pigments and colorants with FORS spectra -...
15 April 2024 6,393 0 View
I'm gonna start to colect biopsy samples from breast cancer and, in order to preserved the RNA for some hours until I can store the sample in the freezer, I'll use some RNAlater. However don't...
29 February 2024 6,147 3 View
Recently, I received peer review feedback that criticized my usage of terms like "neo-tribe" and "neo-tribalism" in a sociological paper. The reviewer found these terms potentially offensive to...
13 August 2023 7,461 0 View
Hi, I generated a stable cell line with a shRNA. However, when I tested the optimal dose of DOX to silence the target gene I obtained a working concentration at 12ug/mL... So, as the majority of...
07 March 2023 4,725 1 View
I'm currently looking for a pH indicator that can be excited in the VIS range (610-630 nm) with emission in NIR range (760-790 nm). I don't have any constrain about the solvent
17 November 2022 6,966 0 View
I am performing mcmc and ss analyzes on MrBayes, with morphological characters, but the ESS are very low. I have already tried to increase the temperature of the swaps and increase the number of...
25 March 2020 4,302 0 View
Hi, I would like to know if anyone has expanded CD8 T cell clones with feeders and check PD-1 kinetic of expression. Is it similar to expression on CD8+ cells stimulated with anti-CD3/CD28 beads?...
18 March 2020 6,700 0 View
The Greater Cincinnati Well-being Observation Tool developed by Clarissa Rentz (2005).
15 March 2020 2,728 3 View
Dear all, Currently, we are trying to produce hydrogen and methane by anaerobic digestion of biodiesel waste (glycerin) at thermophilic temperature (55 °C/ 131 °F). However, as soon as...
10 March 2020 6,805 11 View
Hi everybody, I am struggling to get the TOP flash assay to work using 293T cells. I have tried adding 10 ug of recombinant Wnt3a as positive control and also using Wnt3a conditioned media but I...
14 January 2020 8,583 9 View
Hello, I am currently having problems with RNA extraction. I am using mouse liver (C57BL6J), and I have extracted RNA from mouse liver before. Before this experiment, my final RNA pellets were...
11 August 2024 7,082 3 View
I would like to understand potential safety concerns while handling SEB in the lab. Especially while working in animal house facility. Would like to know precautions for handling. Sigma MSDS...
07 August 2024 6,034 3 View
Hi! So i attempted to understand a novel protein behavior towards heat application by analyzing its secondary structure change. I subjected the protein to a thermal denaturation analysis using...
06 August 2024 1,989 3 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I need to lyse and homogenise mouse tissues. So far I have experimented with liver and lung tissue, using matrix D. The liver was completely lysed with 6 repetitions of the standard program, but...
31 July 2024 9,186 0 View
Dear All, I am trying to transfect a pCDNA3.1 vector containing my gene of interest. The purpose is to figure out the localization of the protein of interest. I have fused the protein with GFP on...
31 July 2024 9,892 4 View
Hello , I established a stable cell line expressing GFP tagged to a centrosomal gene having G418 drug selection marker. I validated the stable line by IFA and Western blotting, results are fine....
29 July 2024 5,007 0 View
I want to do 2,3-butanediol dehydrogenase(BDH) enzyme purification to confirm its activity for 2,3-butanediol. Before that, I need to confirm which N or C terminal tagging is better for enzyme...
28 July 2024 366 3 View
I cannot confirm cell surface protein expression by flow cytometry even after transfection and antibiotic selection of cells. Does it take long for proteins to express on the cell surface? the...
28 July 2024 3,178 3 View
It has been long known that EDC carbodiimide and NHS easter's half life agains hydrolysis are highly related to pH. However, very surprisingly, not yet found a paper giving a table/chart of their...
25 July 2024 8,738 0 View
