I want to isolate DNA from whole blood using the attached protocol.
Sorry, but have you carefully read the paper you attached?
The recipe is in there:
TKM 1:
10 mM Tris-HCl pH 7.6,
10 mM KCl,
10 mM MgCl2 and
2 mM EDTA.
TKM 2:
10 mM Tris-HC1 pH 7.6,
10 mM MgCl2,
0.4 M NaCl and
2 mM EDTA (TKM2).
Thank you ...
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kindly reply me. Thanking you in advance.
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