Sai Krishna Srimadh Bhagavatham

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Questions related from Sai Krishna Srimadh Bhagavatham

Is there anyone who is working on the chondrosarcoma cell line - SW-1353?

Dear All, Is there anyone who can lend me the chondrosarcoma cell line SW-1353?

04 July 2015 6,954 0 View

How many cells can be seeded for doing SOD and Catalase assays in Human Articular Cartilage cells that are grown in Tflasks?

Hi All, I want to do enzyme assays (SOD and Catalase) in chondrocytes from human articular cartilage. Please suggest me how many cells to be seeded in a petriplate or t75 flasks. I tried doing...

13 February 2015 8,393 3 View

How many days can metabolite stock (of ATP, Adenosine, VPA) made for an MTT assay be used?

Dear All, I am currently doing an MTT assay with different compounds like ATP, Adenosine and VPA. And I need to treat the cells for three days. Can I use the compound stocks made on the first day...

06 February 2015 2,502 4 View

How much water needs to be added to Adenosine Deaminase stock from Worthington to make the solution?

Hi all, We have recently purchased Adenosine Deaminase (ADA) from Worthington. It was ADA of 250 Units but, there was no instruction as to how much water or buffer needs to be mixed to make the...

21 September 2014 1,606 2 View

Can anyone give me the protocol for checking Adenosine deaminase levels in blood serum?

I am actually working on some autoimmune diseases, where I am planning to screen patients for adenosine deaminase levels. When I referred to literature, it said I need to follow Giusti Method, but...

26 August 2013 4,307 4 View

Can anyone give me a recipe for TKM1 and TKM2 buffers for DNA isolation from whole blood?

I want to isolate DNA from whole blood using the attached protocol.

27 February 2013 2,414 2 View