There are a host of publications on this subject. I have copied a link (Waters applications) which has an example of plasma sample.
Most UV based hplc analysis of aminoacids need either pre-column (which should be easy to perform) or post column derivatization (you need specific accessory equipment for this) of the amino acids for UV detection. There are a number of derivatization reagents reported in literature.
The best way to go is to look for applications notes from HPLC manufacturers and there are a host of them available in the net and then choose the most appropriate for your application
Good afternoon! This is a question I get from time to time... there are a number of kits available, that use pre-column derivatization with a mass spectrometer or FID. This is really the best way to do it, because you can be sure of the masses present with a mass spec (UV does not have mass data). If you don't have an LC/MS, there are other techniques/detection modes available:
I do agree that for any small molecule analysis (say below 800 daltons MW), ideal HPLC hyphenation for detection is MS. In fact, I prefer double hyphenation HPLC-PDA-MS/MS as this provides comprehensive information on separated constituents However, not all labs have the luxury of having an LC-tandem MS system.
The request above was for info on how to 'run an aminoacid sample in an HPLC with a UV detector". I thought that there is a 'instrument limitation' in Anand's lab and hence the above suggestion.