What could be the reason for different melting peaks within a sample?

I am having a set of primers for a gene. I performed qPCR a week before and got anticipated results. But this time with another sample (same species), did in technical replicates each well shows...

08 September 2019 9,251 5 View

T-RFLP and pyrosequencing?

03 April 2016 4,233 3 View

Does anyone have experience with uneven polymerization in SDS PAGE?

After a long gap I started doing SDS PAGE. This time I came up with a new problem that i found uneven polymerization in the upper surface of the separating gel. I have changed all the components...

04 May 2014 8,849 9 View

How to check the activity of DAB?

In western blotting I confirmed that the proteins were transferred from the gel to the membrane and was sure that the antibody was functional, but then there is no color formation when adding the...

04 May 2013 1,615 4 View

How to isolate protein for expression studies?

I have treated the cells with complex and after a period of time I am isolating the protein for expression studies. During this step should I isolate protein from live cells alone or both the...

02 March 2013 4,181 4 View

During silver staining I am unable to stain the gel - it remains blank as if it were freshly cast. How can I overcome this problem?

I have isolated a protein from cell lines and I want to confirm the presence of the protein by Bradford reagent. But when trying to silver stain, I am unable to stain the gel - it remains blank...

01 February 2013 5,303 33 View

I need to image mesoporous silica nanoparticles that are in ~100 nm size and do high resolution TEM to look at mesopores. what grids should I use?

Need to image mesoporous silica nanoparticles using the TEM. Also, need high resolution TEM images to see the mesoporous structure. Kindly suggest what kind of grids to use. Thanks, Shatadru

02 March 2021 1,787 2 View

Can chitosan alone can act as a reducing agent for Cu(0) nanoparticle sythesis?

[Synthesizing Cu nanoparticles without using chemical reductants]

01 March 2021 8,359 1 View

What are the new 2D nanomaterials were used for Silver nanoparticles stabilization?

In the stabilization of AgNPs are vital in current research interest. However, some of the 2D nano-materials like graphene oxide, MoS2, etc... previously used to stabilize it. But still they shown...

01 March 2021 2,207 3 View

What is the state of the drug inside solid lipid nanoparticles (SLNs)?

I am a little confuse with the mechanism of solid lipid nanoparticles (SLNs). The main point of encapsulating drugs into SLNs is because some drugs have low solubility in water. Therefore, SLNs...

01 March 2021 3,703 3 View

How to check the uv-vis absorption of nanoparticle by surface plasmon resonance?

I try to check the uv-vis absorption of Indium nanoparticle. Now I am measuring in solution, can I measure it in the form of a film?

01 March 2021 930 3 View

Apart from Nanosilica and CNT's what are the other nanomaterials readily available to use in concrete mixture?

Nanotechnology in concrete materials

01 March 2021 3,379 3 View

Distribution of particles in metal matrix?

How would define the "good" and "poor" distribution of particles within the matrix. Is there a quantitative solution to assess the distribution of the particles, which then having denotation of...

28 February 2021 7,397 8 View

What is the density for pva electrospining?

I'm Juan Carlos, a high school student who is working with electropinning, I made an electropinning machine with a 21g needle and a 30kv high voltage source and the distance between my needle is...

28 February 2021 298 3 View

How can I calculate the rdf of water molecules around 'Na' ions that are closest(adsorbed) to the nanoparticle(solute)?

Hi, I want to calculate the radial distribution function for water molecules around the Na+ ions adsorbed on the surface of a spherical nanoparticle using the gromacs trajectory dynamically? For...

28 February 2021 5,650 1 View

LEDs on Vis Light photo degradation of MB water sol via N doped TiO2 in a mini flume?

Good day to all, I have no experience with light emitting diodes. I am building a glass mini flume to demonstrate the photo degradation of Methylene Blue in water running over self cleaning...

26 February 2021 4,026 5 View