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After a long gap I started doing SDS PAGE. This time I came up with a new problem that i found uneven polymerization in the upper surface of the separating gel. I have changed all the components...
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In western blotting I confirmed that the proteins were transferred from the gel to the membrane and was sure that the antibody was functional, but then there is no color formation when adding the...
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I have treated the cells with complex and after a period of time I am isolating the protein for expression studies. During this step should I isolate protein from live cells alone or both the...
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I have isolated a protein from cell lines and I want to confirm the presence of the protein by Bradford reagent. But when trying to silver stain, I am unable to stain the gel - it remains blank...
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