Iam looking for the ADCC experiment can anyone explain me the protocol of the experiment and importance of this experiment
Search Youtube for "ADCC Youtube", you will finde many videos. Go for lectures and courses to learn about the principle and application. Several companies also have released videos about their specific ADCC assay kits. Which one is best for you depends on your context -the exact questions you are addressing and the equipment you have in your lab.
https://www.youtube.com/watch?v=MT31FlFw7mU
https://www.youtube.com/watch?v=H3h0qwHHgBc
https://www.youtube.com/watch?v=N3L4kQqsGPQ
https://www.youtube.com/watch?v=H3I0GcsP4h4
https://www.youtube.com/watch?v=77y9d5hVXh8
https://www.youtube.com/watch?v=H3I0GcsP4h4
https://www.youtube.com/watch?v=mkiBEl_fZyE
Dear Pradyumna Iragavarapu
Fc-mediated immune effector functions are an important part of antibody natural functions and it is happen trhough the binding of he constant region of the human antibody with different Fc receptors expressed in the surface of some effector cells present in different cells (eg FcyRIIIa in Nk FcγIIa in macrophages for ADCP),
In recent years the enhancement or elimination of the Fc-effector function has led to a growing interest in Fc-engineering, to give antibodies specific mechanisms of action and therapeutic properties.
If in some cases, the complete elimination of the binding to Fc gamma receptors (FcγR) was highly desirable to avoid unwanted inflammatory responses, in other cases engage and activate specific classes of FcyRs by engineering or afucosilation of the Fc has led to the development of more potent antibodies.
you can have an overwiev of this be reading:
- https://www.ncbi.nlm.nih.gov/pmc/articles/PMC8396266/
- https://ita.promega.com/products/reporter-bioassays/fc-effector-activity-bioassays/adcc-bioassays/?catNum=G7015
- https://pubmed.ncbi.nlm.nih.gov/29733746/
best regards
Manuele
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