Do we have any other online tools to determine the equal volumes of the sample, or do we need to just do normal math?
I want to do disc modelling from experimental data. How to identify different parameters involved in disc brake induced vibration.
26 September 2023 7,850 0 View
Iam looking for the ADCC experiment can anyone explain me the protocol of the experiment and importance of this experiment
15 June 2023 8,212 2 View
can any one help me out with any wet lab experiments that they have performed on HHLA2 immune marker which has significant survival association with tumor microenvironment. I have done some data...
15 June 2023 9,012 0 View
I have Hazard Ratio and 95% CI, I tried to get standard error using Comprehensive meta analysis tool, the log value given in the tool seems misleading. Is there a manual way to calculate Standard...
30 August 2018 4,998 4 View
Hi, I am doing MIP optimization on conveying capacity increase of pellets by proper silo routing and unloading. I need to specify a constraint saying that once a silo is selected it needs to be...
17 July 2016 6,306 8 View
Hi everyone! I have a silo(storage capacity) which is being filled with solid pellets. When the silo is fully filled, the binary variable has to be switched from 0 to 1. How can it be done...
21 June 2016 6,353 6 View
In the process of investment casting, either silica or alumina based mixes are used as injected ceramic cores to provide complex cooling passages in the casting. For low temperature DS (direction...
20 September 2015 5,928 3 View
I am designing a Low Noise Amplifier (LNA) for Ku-band(12GHz). For that I am using HJFET transistor. I am looking for the biasing circuit which can be useful to block RF signals of GHz range but...
28 October 2014 8,498 7 View
Molecular epidemiological studies with the aim to establish dose-response relationship have failed to demonstrate direct-association of acute exposures (exposure to a chemicals once in lifetime,...
21 September 2014 5,369 6 View
We have observed significant reduction in mtDNA content, elevated ROS levels, depleted anti-oxidant enzyme activities leading to up-regulation of p21 and down-regulation of cyclin D1, following...
12 August 2014 8,052 2 View
I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...
09 August 2024 9,621 0 View
Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...
09 August 2024 2,824 3 View
I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...
07 August 2024 7,535 4 View
Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...
07 August 2024 8,106 4 View
Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...
06 August 2024 641 2 View
I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...
04 August 2024 9,913 7 View
I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...
04 August 2024 1,200 2 View
Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...
04 August 2024 7,282 2 View
I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...
03 August 2024 3,851 1 View
Hello, if you made a transwell assay where you incubate the cells with a nanoparticle-encapsulated drug and considering that in the oposite compartment you'll have both the free and encapsulte...
03 August 2024 2,730 1 View