What is the standard protocol for flow cytometry?

Can anyone guide me, What are all the things i've to concentrate more while preparing the samples for the detection of membrane bound proteins by indirect staining in flow cytometry?

03 April 2013 4,338 1 View

Can anyone suggest how to do normalization in western blot bands?

While studying expression of a single protein from different cell sources by western blot technique how to calculate changes in the expression of protein by normalization?

02 March 2013 4,893 5 View

How to avoid non specific band in western blotting while detecting with Cy3 & Cy5 linked fluorescent antibodies.

My primary antibody is a poly clonal antibody. Even though I'm using 5% skimmed milk for blocking and 0.1% PBST at three times for five minutes each for washing, I' still getting non-specific...

01 February 2013 5,605 6 View

What percentage of SDS PAGE gel is preferable for running 85 KDa to 55 kDa protein for western blotting ?

Now I'm using 12 % SDS PAGE, I'd like to increase a gap between 85 kDa and 55 kDa protein bands to get a better result, Can anyone give me some suggestions about what percentage of SDS PAGE gel I...

01 February 2013 2,857 9 View

Can we classify HV and LV lines based on line length?

I have dataset which shows the length of power lines. I need to classify the lines based on the line length. Is there a rule to classify the High voltage (HV) and low voltage (LV) lines based on...

03 March 2021 4,116 4 View

Why does blocking with my immunogenic peptide cause nuclear fluorescence in ICC?

Hi all, I am validating a polyclonal antibody raised against human ANT4, through WB and ICC. In most of my controls, the antibody shows very little off-target binding, but when I pre-incubate the...

03 March 2021 5,999 3 View

How to increase a model accuracy ?

dear community, my model is based feature extraction from non stationary signals using discrete Wavelet Transform and then using statistical features then machine learning classifiers in order to...

03 March 2021 6,994 5 View

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02 March 2021 3,060 3 View

Does Napabucasin soluble in dichloromethane and in ethanol?

I need to prepare a solution of Napabucasin (inhibitor of cancer cell stemness) in dichloromethane. The drug is expensive, so maybe somebody already tried to do it. If it is soluble, what is the...

02 March 2021 9,945 3 View

Help request in CST microwave?

im working now on TPV solar cells and metamaterials and i realy need our help in CST microwave i have a tpv cell simulation and i want to extract particullarly the absorbtion of only the active...

02 March 2021 2,246 2 View

Which SAR dataset to use for generating coherency matrix and performing target decomposition (Description) ?

Working on chandrayaan-2 DFSAR data, there are three datasets available: 1) Slant range image data product: The slant range complex image file. Each pixel is represented by two 4-byte floating...

02 March 2021 8,481 3 View

Issues with Permeabilization during Flow Cytometry?

Hello Everyone. Currently I am working to characterize macrophages in the myocardium after ischemia-reperfusion injury in rats. Due to the low total cell number isolated from rat hearts I can...

01 March 2021 3,867 3 View

Which cell line should we use to perform biological dosimetry experiments?

We are preparing some experiments based on irradiating cells under different conditions in order to evaluate the effects in terms of DNA damage, genetic expression, etc. As our project is...

01 March 2021 3,355 3 View

Is it possible for two human neuronal cell lines to have different gene sequence for the same protein?

I am looking at the ATP1A2 (Sodium/Potassium ATPase alpha subunit 2) in two human neuronal cell lines. Expression levels of this protein seems to be almost equal when detected by one antibody....

01 March 2021 3,607 3 View