Angel Jemima

5 Questions 9 Answers 0 Followers

Questions related from Angel Jemima

What is the standard protocol for flow cytometry?

Can anyone guide me, What are all the things i've to concentrate more while preparing the samples for the detection of membrane bound proteins by indirect staining in flow cytometry?

04 April 2013 4,372 1 View

Can anyone suggest how to do normalization in western blot bands?

While studying expression of a single protein from different cell sources by western blot technique how to calculate changes in the expression of protein by normalization?

03 March 2013 4,933 5 View

What percentage of SDS PAGE gel is preferable for running 85 KDa to 55 kDa protein for western blotting ?

Now I'm using 12 % SDS PAGE, I'd like to increase a gap between 85 kDa and 55 kDa protein bands to get a better result, Can anyone give me some suggestions about what percentage of SDS PAGE gel I...

02 February 2013 2,895 9 View

How to avoid non specific band in western blotting while detecting with Cy3 & Cy5 linked fluorescent antibodies.

My primary antibody is a poly clonal antibody. Even though I'm using 5% skimmed milk for blocking and 0.1% PBST at three times for five minutes each for washing, I' still getting non-specific...

02 February 2013 5,639 6 View

Can anyone explain me about the major differences between LAD-2 and LUVA mast cell lines?

Both LAD-2 and LUVA are human mast cell lines and its already known that, both can able to express FcεRI receptor on their surface. LUVA is fast growing cells and had a increased life span...

01 January 1970 1,774 2 View