The simplest way of getting rid of primer dimer is to use a hot start polymerase. If this is not possible just use less primer ( try 1/2, 1/4 and 1/8 the amount you are using now)

Thank you Paul Rutland

as you have suggested me above I already tried with different concentrations but still I am getting the same band, so if someone can tell the exact procedure like how much DNA concentration, any positive control and how would the banding pattern appear n that positive control and other PCR conditions would help me solve this problem

Thank you

Hi,

did you test the PCR also with pure DNA e.g. E.Coli? If not it could be PCR inhibition which favors Primer dimers.

So my first step would be going this way and if Inhibition is the isse switch to inhibitor resistant mixes and deidcated sample prep with inhibitor removal

Good luck

Sven

OK Sir, thank you i will try them

Hello Dr@ Avinash

I do ERIC PCR and have the same problem of primer dimer ؟ did you find the solution of this proplem ?