Avinash Ll

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Questions related from Avinash Ll

Can anyone explain in detail how to put a ERIC PCR?

I did ERIC PCR with ERIC1 and ERIC2 primer at 52 annealing temperature and I got a primer dimer and I did gradient PCR from 50 to 60 annealing temperature and still I got a primer dimer. can...

10 March 2020 9,115 5 View

Can some one help me with making a multiplex PCR primer mix?

I have designed a PCR primer of primerA (F), primerB (F) and primerAB (R), can anyone explain how to mix this primer as a one mixture to add to my master mix for PCR amplification of gene of interest

01 January 1970 5,069 3 View