Can anyone explain how to isolate mast cells from cord blood?

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Rohit Saluja

Please find the protocol for CBMC in this paper

Angel Jemima

Thank you Rohit and Bishwas for your suggestions, I have an another question. Is there any method is available to purify CD 34+ cells other than CD34 MicroBead kit?

Lepelletier Yves

Cell sorting by facs

Salme Suhana

hi everyone, just want to ask can I use other type of interleukin instead of IL-3? for example IL-2 for growing bone marrow derived mast cell?

Thank you in advance :-)