Angel Jemima

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Questions related from Angel Jemima

Can anyone explain how to isolate mast cells from cord blood?

I'm planing to isolate mast cells from cord blood using a hematopoietic medium supplemented with scf 100 ng/ml, IL-6 (50 ng/ml), IL-3 (1 ng/ml) and confirmation of mast cells by toluidine blue...

22 August 2013 108 4 View

Can anyone suggest how to do normalization in western blot bands?

While studying expression of a single protein from different cell sources by western blot technique how to calculate changes in the expression of protein by normalization?

11 March 2013 7,002 4 View

What is the hope for development of Histamine H4 Receptor antagonist in the treatment of asthma?

Antihistamine drugs are mostly used in the treatment of allergy and inflammation. Any evidence is there for recently discovered Histamine H4 receptor related research for the treatment of Asthma.

11 March 2013 902 1 View

What percentage of SDS PAGE gel is preferable for running 85 KDa to 55 kDa protein for western blotting ?

Now I'm using 12 % SDS PAGE, I'd like to increase a gap between 85 kDa and 55 kDa protein bands to get a better result, Can anyone give me some suggestions about what percentage of SDS PAGE gel I...

25 February 2013 733 7 View

Where to get LAD2 cell line?

I need to purchase LAD2 cell lines for my research work. Does anyone know which company or organizations can provide it apart from Dr. Metcalfe at NIH?

11 August 2012 1,967 0 View