Can anyone can suggest the antifungal agents which can be used for the enumeration of bacterial culture from insects using Luria or nutrient broth?

More Richu Singla's questions See All

What is the meaning of 4 in the formula of TAN (Titrable Acid Number)?

Respected Researchers Kindly explain the below mentioned formula for the calculation of TAN. I am not able to understand 4 in this formula. Thanks in advance

05 March 2024 5,545 1 View

How to calculate total magnetic moment of a material when I have magnetic spin magnetic moment value for individual atom of that material ?

I have calculated the Spin Magnetic moment for all individual atoms of a 2-D material via simulation. Is the total magnetic moment of this 2-D material just the sum of all individual magnetic...

22 December 2022 6,439 9 View

Isolation of DNA from PLASMA and PCR amplification?

Isolating DNA from plasma using a column. DNA concentration on nanodrop is 5ng/ul. Unable to see any band in gel after 3-5 ul load no result in PCR amplification? what can be the reason?

17 November 2022 6,552 8 View

How can we call TRNSYS model with MATLAB code for parametric analysis and optimization?

I want to call TRNSYS model with MATLAB code for parametric analysis and optimization. Can anyone help me with the syntax for the same?

11 August 2022 8,857 0 View

How to separate phenolic compounds from simple sugars from fruit sample using column chromatography?

Solid phase extraction column chromatography

04 August 2021 9,549 5 View

Likert scale and alpha value ?

I am planning to get a questionnaire filled from the students where they will rate their skills like communication skills, creative skills etc from 1 to 5. Can I call it Likert scale and can I use...

12 July 2021 2,235 2 View

What are the latest guidelines for the award of Ph.D degree and allocation criteria for the supervisors?

Pls share the latest guidelines of UGC for the award of Ph.D degree by any of the Govt or Private universities.

08 July 2021 1,882 3 View

Manual to start working with QCA designer tool ????

Dear All, I am beginner in the QCA circuit designs. I need to learn wirking with QCA designer tool. I searched many items on google but no manual or any help i found where i can learn it step by...

06 August 2018 444 3 View

In a single phase low voltage battery charger the initial battery charging current is negative and battery is discharging during that interval?

The AC source voltage is 120V. The AC -DC full bridge boost converter and DC-DC buck converter is used.The controller for AC-DC converter consists of PI and PR controller but PR controller is not...

06 April 2018 8,532 0 View

Hello everyone, i want to prepare standard protein solution with concentration 100 microgram/ml. How would i prepare?

I want to do protein estimation by Lowry's Procedure. For standard, i have to prepare standard curve with bovine serum albumin (100 microgram /ml) . I have to prepare a standard curve upto 150...

17 March 2018 4,106 4 View

Why Do TDS and EC Increase with Larger Wastewater Volumes, While BOD and COD Decrease?

I have carried out MFC experiments on three different volumes, 50, 500 and 1000 mL of wastewater. Results after MFC treatment shows that TDS and EC are more in larger volumes of water i.e. TDS and...

09 August 2024 9,621 0 View

Could it be a cell culture contamination?

Hello everyone! I observed in my culture (htert-RPE1 cells) an orange- red particle at the bottom of the dish. It is visible to the naked eye as a very very small red dot. Could it be a...

09 August 2024 2,824 3 View

Is there any way to quantify bacterial and fungal cells in their mixed culture?

I am working in fungal fermentation of soybean meal and there is bacterial growth in them at times. I am trying to quantify fungal cell counts and bacterial cells; but I haven't been able to do at...

07 August 2024 7,535 4 View

How to normalize and take the significance of the MTT OD values with 3 replicates for the same cell-line?

Hi, I have a question about normalizing the MTT OD values for doing the statistical analysis. So, if we have 3 different plates and we call them 3 different replicates, so, first we would...

07 August 2024 8,106 4 View

Why activated CAR-Jurkat cell could not kill targets?

Previously when I co-coluture anti-CD19(FMC63) CAR-Jurkat with Raji with E:T=5:1, Jurkat can eliminate Raji in 24h. However, when I test another CAR construct, although I can dectect totally CD69...

06 August 2024 641 2 View

How to isolate lymphocytes from mouse spleen?

I have tried several times to isolate lymphocytes from mouse spleen, but all attempts have been unsuccessful. I tried most available protocols. I used different dissociation media (HBSS with Ca...

04 August 2024 9,913 7 View

How to Add Missing Water Molecules in Protein-Membrane Simulations?

I have protein-membrane simulations (PDB, PSF, DCD) and have noticed that water molecules near the protein are not visible in the simulations. How can I fix this issue? Is there a way to place the...

04 August 2024 1,200 2 View

Does long-term culture of murine Monocytes result in macrophage differentiation?

Hi, I am isolating monocytes from the bone marrow using the Mouse Monocyte EasySep kit. I want to treat these cells and monitor expression of specific markers over the course of 10 days. I will...

04 August 2024 7,282 2 View

Good Day, can I ask for your help on the questionnaire that I want to adopt?

I am currently looking for a questionnaire that relates with my study which is The Effectiveness of Organizational Culture towards employee retention rates

03 August 2024 3,505 6 View

To perform transfection with DharmaFECT Duo in AGS cells. Could you tell me what the ideal concentration is to avoid significant cytotoxicity?

I would like to perform transfection with the reagent DharmaFECT Duo (Horizon) on the AGS cell line. Could you please inform me of the optimal concentration to use without causing cytotoxicity in...

03 August 2024 3,851 1 View