Hello everyone
I am reading some papers and I am confused.
In the disc diffusion method for testing antimicrobial activity of substances, the antimicrobial substance is absorbed on a paper disc which is applied on a culture media inoculated with the targeted microorganism.
Now my question:
After incubation, should the diameter of the paper disc be taken in consideration while measuring the zone of inhibition or not?
All your contributions are welcome.
Please refer to Clinical Laboratory Standard Institute (CLSI) practices when doing antibiotic sensitivity testing. These practices are accepted internationally and are adhered by most clinical laboratories, especially those with ISO 15189
No, you don't usually measure the diameter of the disc, just the diameter of the zones of inhibition. Please see section 7 of the BSAC guide:
you may follow the procedure as described by @ Micheal. That is most self-explanatory.
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