18 September 2015 3 8K Report

Found in some articles that Carrier RNA enhanced the yield of DNA from a variety of sources, so I tried for the first time during my DNA extraction from tiger faecal samples, but some unusual results appeared in my mitochondrial PCR. I am using Qiagen Stool Mini Kit and got the lyophilized carrier RNA from Qiagen as well. If anyone put few lines on the dilution of the carrier RNA (whether RNase free water or buffer from Qiagen?) and at which step to add the same during extraction process -- would be great.

Regards, Aziz

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