I am using HEPG2 cell line, with low glucose medium and using HEPES and sodium bicarbonate for maintenance. please let me know if i am planning for further experiments will HEPES interfere with flow experiments and other ???
It has been a number of years since I last worked with HepG2 cells, but I have used both Sodium Bicarbonate or HEPES buffer. If i recall correctly, I used HEPES more often in microfluidic devices and plates for longtime studies on the microscope. It seemed to help maintain the conditions better. It has been 10 years since I worked with these so hopefully someone with more recent experience will answer.
Dear Sanjay,
Whenever working with cell line, please check the ATCC http://www.atcc.org/ for culture specifications, characteristics, doubling time, etc.
For example: Hep G2 [HEPG2] (ATCC® HB-8065™) cells
Complete Growth Medium The base medium for this cell line is ATCC-formulated Eagle's Minimum Essential Medium, Catalog No. 30-2003. To make the complete growth medium, add the following components to the base medium: fetal bovine serum to a final concentration of 10%.
More information on this cell line here: http://www.atcc.org/Products/All/HB-8065.aspx#culturemethod
Good luck!
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