I want to elicit my cultures using MJ, which concentrations are being generally used. and how to prepare MJ stock solution? and is it necessary to filter sterilize it before adding to medium?
Methyl Jasmonate generally dissolved in ethanol and to use MJ in medium it need to prepare stock solution.
Here is calculation for stock solution preparation.
For 250 ml of 100 µM stock solution
224.3 g / Mol x 100 µMol/L x 1 Mol/106 µMol x 1L/ 103 ml x 250 ml = 0.0056 g mol
Thanx, but mine is already solution (purchased from Sigma aldrich) so can i use it directly in medium? by calculating the concentration using M1V1=M2V2 (main stock would be 1M- the purchased one)?
I think you must use different concentrations to produce the best results
Yeah but can I consider my solution as 1 Molar and accordingly calculate and add different concentrations?as mine is already a solution.
You can find the density from place where you ordered and/or on the package/bottle of the reagent. In sigma aldrich's site it is given that d=1.03 g/mL
Which means, in 1L of solution you have m/1000mL=1.03g/mL m=1030g
Now, to get 1mM solution of 50mL you should have 224.3/(1000/(1000/50))=11.2mg
11.2mg will correspond to 1.09x10^-5 of the 1L solution, which wi
tres of solution. Considering the fact the it is %95 solution, you should have 10.9/0.95=11.5microliters taken from stock solution and filled with the solvent to fill 50mL solution.
I hope calculations are clear.
Good luck
I am thinking of the same thing now. I bought MJ 85% from Sigma. A liquid. That is an oil or neat oil, or pure oil, or oil without water. The suggested solvent is ethanol. As oil, to make it together with water, we need to add an emulsifier.
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